A medium for promoting rooting of tetraploid paulownia tissue cultured seedlings and its application

A rooting medium and medium technology, applied in the biological field, can solve the problems of low rooting rate and irregular rooting of tetraploid Paulownia tissue culture seedlings, and achieve the effects of improving the survival rate of seedlings, promoting rooting, and high rooting rate

Active Publication Date: 2021-06-01
SHANDONG FOREST SCI RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: how to provide a medium formula and application for promoting rooting of tetraploid paulownia tissue culture seedlings, so as to solve the problems of low rooting rate and irregular rooting of tetraploid paulownia tissue culture seedlings in factory cultivation

Method used

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  • A medium for promoting rooting of tetraploid paulownia tissue cultured seedlings and its application
  • A medium for promoting rooting of tetraploid paulownia tissue cultured seedlings and its application
  • A medium for promoting rooting of tetraploid paulownia tissue cultured seedlings and its application

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Experimental program
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Effect test

Embodiment 1

[0021] Under sterile conditions, cut off the subcultured plant height of about 2cm, with more than 2 pairs of leaves with budding stems, cut into individual plants, and inoculate them into the rooting medium. The composition of the rooting medium is MS+IBA0. 1mg / L+IAA 0.05mg / L+NAA0.1mg / L+agar 4.0g / L+20g / L sucrose (pH 5.8), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. Cultivate for about 12 days to form root primordia, and grow tender roots from the root primordium in about 14 days. After 20 days of cultivation, seedlings can be hardened and transplanted. The number of roots is 5-7, the root length is 0.5-1.5cm, and the rooting rate is 100%.

Embodiment 2

[0023] Under sterile conditions, cut off the subcultured shoots with a height of about 2 cm, with more than 2 pairs of leaves, cut into individual plants, and inoculate them into the rooting medium. The composition of the rooting medium is MS+IBA 1.5 mg / L+IAA 0.5mg / L+NAA 0.3mg / L+agar 4.5g / L+20g / L sucrose (pH 5.8), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. Cultivate for about 10 days to form root primordia, and grow tender roots from the root primordia in about 11 days. After 20 days of cultivation, seedlings can be hardened and transplanted. The number of roots is 5-18, the root length is 1.5-3.0cm, and the rooting rate is 100%.

Embodiment 3

[0025] Under sterile conditions, cut off the subcultured shoots with a height of about 2cm and more than 2 pairs of leaves, cut into individual plants, and inoculate them into the rooting medium. The composition of the rooting medium is MS+IBA 4.0 mg / L+IAA 2.0mg / L+NAA 2.0mg / L+agar 6.0g / L+20g / L sucrose (pH 5.8), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. Cultivate for about 10 days to form root primordia, and grow tender roots from the root primordium in about 13 days. After 20 days of cultivation, seedlings can be hardened and transplanted. The number of roots is 5-12, the root length is 1.0-2.0cm, and the rooting rate is 100%.

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Abstract

The invention belongs to the technical field of plant medium formulations, in particular to a medium formulation for promoting rooting of tetraploid Paulownia tissue culture seedlings. The medium is added with 0.1-4.0mg / L indolebutyric acid, 0.05-2.0mg / L indoleacetic acid, 0.1-2.0mg / L naphthaleneacetic acid, 4.0-6.0g / L agar, 20g / L sucrose composition. The formula can significantly promote the rooting of tetraploid paulownia tissue culture seedlings in a bottle. The rooting rate reaches 100%. The invention effectively solves the problem that the tetraploid paulownia tissue culture seedlings are difficult to root.

Description

technical field [0001] The invention relates to a culture medium for promoting rooting of tetraploid paulownia tissue culture seedlings and its application, belonging to the field of biotechnology. Background technique [0002] Paulownia is a plant of the genus Paulownia in the family Scrophulariaceae. It is native to my country. It has no flying catkins, beautiful trees, and a long history of cultivation. It is naturally distributed in 25 provinces, municipalities, and autonomous regions in my country. It is an important fast-growing timber and native tree species for landscaping in my country. The leaves, flowers, fruits and bark of Paulownia can be used as medicine, and the leaves and flowers of Paulownia can be used as feed. Paulownia wood is high-quality and high-grade, corrosion-resistant, acid and alkali resistant, wear-resistant, easy to process, easy to carve, long fiber, light color, easy to dye, fine texture, natural color, beautiful pattern, uniform structure, go...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 毛秀红刘欣闫少波毛欣华辉王迎李猛仲伟国亓玉昆于振旭杜辉赵青松
Owner SHANDONG FOREST SCI RES INST
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