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Differentiation promoting reagent YYQ1 capable of obviously promoting differentiation of mouse C2C12 myoblasts through external addition

A technology for myoblasts and mice, applied in the field of cell biology, can solve the problems of non-differentiation, crazy cell growth, poor cell state, etc., and achieve the effects of simple production, low cost and strong operability

Inactive Publication Date: 2020-07-03
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Application Information

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Problems solved by technology

[0003] In order to overcome the problem that the cell state is often not good, the cells just keep growing wildly, and no differentiation is induced in the process of mouse C2C12 myoblast culture experiment, the present invention provides a method that can significantly promote the growth of mouse C2C12 through the addition of exogenous sources. The differentiation-promoting reagent YYQ1 for muscle cell differentiation, which can significantly promote the differentiation of mouse C2C12 myoblasts through the addition of exogenously C2C12 cells can obtain better differentiation efficiency, promote the differentiation of C2C12 cells, and finally achieve the purpose of significantly promoting the differentiation of mouse C2C12 myoblasts after the addition of exogenous sources. The differentiation-promoting reagent YYQ1 provides a basis for promoting animal muscle differentiation. Retinol promotes muscle differentiation and has great practical application value

Method used

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  • Differentiation promoting reagent YYQ1 capable of obviously promoting differentiation of mouse C2C12 myoblasts through external addition
  • Differentiation promoting reagent YYQ1 capable of obviously promoting differentiation of mouse C2C12 myoblasts through external addition
  • Differentiation promoting reagent YYQ1 capable of obviously promoting differentiation of mouse C2C12 myoblasts through external addition

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Embodiment 1

[0018]As shown in the figure, the composition of the differentiation-promoting reagent YYQ1 that can significantly promote the differentiation of mouse C2C12 myoblasts through exogenous addition is: 10 μM retinol (Retinol, ROL for short). The experimental procedures related to the differentiation activity of C2C12 myoblasts are as follows:

[0019] 1. C2C12 cell culture and retinol addition experiment:

[0020] The mouse C2C12 cell line was purchased from Shanghai Sangong (Shanghai, China). In order to study the effect of ROL on the differentiation of mouse C2C12 cells, the cells were subcultured and cultured in six-well plates, and the C2C12 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum and penicillin-streptomycin. cultured in liquid. When the cell density reached 80%-90%, the differentiation medium containing 2% horse serum and penicillin-streptomycin was replaced to induce differentiation, and the final concentrations o...

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Abstract

The invention discloses a differentiation promoting reagent YYQ1 capable of obviously promoting differentiation of mouse C2C12 myoblasts through external addition, and belongs to the technical field of cell biology. The differentiation promoting reagent YYQ1 is characterized in that: the component of the differentiation promoting reagent YYQ1 is 10[muM] of retinol, and after the differentiation promoting reagent YYQ1 is added into C2C12 cells cultured in vitro, good differentiation efficiency can be obtained, differentiation of the C2C12 cells is promoted, and finally the aim of remarkably promoting differentiation of mouse C2C12 myoblasts is finally achieved after external addition. The differentiation promoting reagent YYQ1 provides a basis for promoting muscle differentiation of animals, the retinol is used for promoting muscle differentiation, and a high practical application value is achieved. The differentiation promoting reagent YYQ1 has the advantages of simple manufacture, strong operability, low cost and obvious effect.

Description

technical field [0001] The invention relates to a differentiation-promoting reagent YYQ1 which can significantly promote the differentiation of mouse C2C12 myoblasts through exogenous addition, and belongs to the technical field of cell biology. Background technique [0002] Retinol (ROL for short), also known as vitamin A, is a fat-soluble alcohol substance. Retinol plays an important role in maintaining normal visual function, maintaining normal bone growth and development, and promoting cell proliferation and growth. There are many molecular forms of vitamin A, which is an unsaturated monohydric alcohol with an alicyclic ring, including vitamin A1 and vitamin A2 from animal food sources. Vitamin A1 mostly exists in the liver of mammals and saltwater fish, while vitamin A2 often exists in the liver of freshwater fish. Since the activity of vitamin A2 is relatively low, vitamin A is usually referred to as vitamin A1. Vitamin A as used in this application is also vitamin A1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0658C12N2500/38
Inventor 严云勤张闻宇佟慧丽李树峰
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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