Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Specific microsatellite primer composition used for identifying experimental miniature pig variety and method for identifying experimental miniature pig variety

A primer composition and microsatellite technology, applied in biochemical equipment and methods, microbial measurement/inspection, recombinant DNA technology, etc., can solve the problem of primers not having specificity between breeds, time-consuming and labor-intensive results, and limited experimental use of miniature pigs Variety identification and other issues, to achieve the effect of optimizing test conditions and methods of use, high accuracy and reliability

Active Publication Date: 2020-07-03
SHANGHAI ACAD OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the mining of microsatellites in the pig genome is not comprehensive enough, we can only use the microsatellite sequences of other species for homology comparison analysis, and the corresponding primers designed are not specific among breeds, and the identification of breeds is time-consuming and laborious and the results are inaccurate , limiting the identification of experimental miniature pig breeds

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Specific microsatellite primer composition used for identifying experimental miniature pig variety and method for identifying experimental miniature pig variety
  • Specific microsatellite primer composition used for identifying experimental miniature pig variety and method for identifying experimental miniature pig variety
  • Specific microsatellite primer composition used for identifying experimental miniature pig variety and method for identifying experimental miniature pig variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A total of 160 miniature pigs from 4 experimental pig breeds (48 Wuzhishan pigs, 15 Bama pigs, 50 inbred Luchuan pigs, and 47 Tibetan Xiang pigs) were selected, and ear tissue samples from each individual were collected and placed in a In EP tubes with 75% ethanol, the phenol-chloroform method was used to extract genomic DNA, respectively using NanoDrop 2000 (Thermo Fisher Scientific, USA), 1% agarose gel electrophoresis, and gel imaging (Beijing Zhongliwan Biotechnology Co., Ltd., Newbio Gi-1) to detect DNA concentration, OD value and DNA quality, and perform the following operations on DNA with DNA concentration ≥ 50 ng / μL, OD260 / 280 between 1.6 and 1.8, and agarose gel electrophoresis imaging as highlighted bands .

[0039] Fluorescent primers and common primers were synthesized by Shanghai Passino Biotechnology Co., Ltd. The nucleic acid sequences of the primers are shown in Table 1, and the PCR reaction system is shown in Table 2. Among them, the DNA concentration ...

Embodiment 2

[0065] Collect the ear tissues of 10 individual minipigs for the experiment, and use the 10 pigs as individuals to be tested for breed identification, number the individuals as #1-#10, and record the breed information corresponding to each individual number (including: # 1-#3 is Wuzhishan pig, #4 is Bama pig, #5-#7 is inbred Luchuan pig and #8-#10 is Tibetan pig) for comparison with microsatellite identification results. Each individual ear tissue sample was collected and placed in an EP tube filled with 75% ethanol, and genomic DNA was extracted using the phenol-chloroform method. Gel imaging (Beijing Zhongliwan Biotechnology Co., Ltd., Newbio Gi-1) was used to detect DNA concentration, OD value and DNA quality. The imaging of electrophoresis is performed as follows for the DNA in the highlighted band.

[0066] Fluorescent primers and common primers were synthesized by Shanghai Passino Biotechnology Co., Ltd. The nucleic acid sequences of the primers are shown in Table 1, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a specific microsatellite primer composition used for identifying an experimental miniature pig variety and a method for identifying the experimental miniature pig variety. Theprimer composition comprises three pairs of primers. According to the invention, on the basis of microsatellite sequences in the genome of the miniature pig variety, the specific microsatellite sequences and primers among different experimental miniature pig varieties are obtained through verification and screening of population tests; test conditions and use methods of the primers are optimized;and identification of the experimental miniature pig variety is solved.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a specific microsatellite primer composition for identifying experimental miniature pig breeds and a method for identifying experimental miniature pig breeds. Background technique [0002] Experimental miniature pigs are one of the most widely used non-rodent large-scale experimental animals in biomedical research. They have irreplaceable advantages over other experimental animals, and as the most likely donors for xenotransplantation, they have become a research hotspot. Research on miniature pigs And development and utilization are generally concerned by the biomedical circles. There are various breeds of miniature pigs used in experiments. Typical ones in my country include Wuzhishan pigs, Bama pigs, inbred Luchuan pigs, Tibetan fragrant pigs, Tibetan miniature pigs, Banna miniature pigs, Jianbaixiang pigs, Juma miniature pigs, and Wujin pigs. Pigs, cooperat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2600/124C12Q2563/107
Inventor 王洪洋谈永松涂尾龙张莺莺吴华莉曹建国
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com