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Plant regeneration method of Campanula medium

A technology of bellflower and plant, applied in the field of plant regeneration of bellflower, can solve the problems of long germination time, small seeds, slow growth of young leaves and the like

Inactive Publication Date: 2020-07-07
XIAN UNVERSITY OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bellflowers are generally propagated by sowing, but the seeds are small, take a long time to germinate, the young leaves grow slowly, and the growth cycle is long, and they will not bloom until the next year

Method used

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  • Plant regeneration method of Campanula medium
  • Plant regeneration method of Campanula medium
  • Plant regeneration method of Campanula medium

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Embodiment Construction

[0035] 1. Acquisition of sterile materials: Soak the bellflower seeds with sterile water, and after the seeds grow radicles, use 0.2% H2O with a mass concentration of 0.2% to expose the radicles. 2 o 2 After sterilizing for 2 minutes to 3 minutes, inoculate on water agar medium (agar content is 6g / L) to germinate to obtain seedlings with cotyledons and young true leaves, and cut off the cotyledons or young true leaves as explants for later use;

[0036] Or the bellflower seed is sowed in the seedling pot that the mixed substrate of 85wt% peat, 10wt% perlite and 5wt% vermiculite is housed and germinates, obtains the seedling that has cotyledon and young tender true leaf, uses seedling in ultra-clean bench Soak in 75% alcohol for 30 seconds, rinse once with sterile water, sterilize with 10% NaClO for 5 minutes, rinse with sterile water for 4 to 5 times, dry the surface with sterile absorbent paper, cut The lower cotyledons or young true leaves are used as explants for later use...

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Abstract

The invention discloses a plant regeneration method of Campanula medium. The method comprises the following steps: firstly, preparing an explant for standby application; secondly, placing the explantin a culture medium, and culturing the explant in a dark condition to obtain calluses; thirdly, carrying out subculture on the calluses, and then transferring the calluses into light for culture; fourthly, transferring the calluses subjected to green turning to a bud differentiation culture medium for culture, separating adventitious buds, inoculating the adventitious buds to a rooting culture medium for culture, and obtaining tissue culture seedlings; and fifthly, transplanting the tissue culture seedlings into a matrix, and growing the tissue culture seedlings in a greenhouse to obtain a Campanula medium plant. The method is simple and easy to implement, low in cost and convenient to manage. Compared with existing seed propagation, the method overcomes the defects that cultivation management is not easy after sowing, the germination rate is low in artificial cultivation, and few seedlings are formed. Meanwhile, the growth cycle can be greatly shortened, the reproduction rate is increased, and high-quality seedlings which are consistent in specification and convenient to cultivate and manage are provided in a short time.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a plant regeneration method of bellflower. Background technique [0002] Campanula medium L., also known as bell flower and tile flower. Its plant shape is strong and its flowers are bell-shaped like wind chimes. It can be used not only as a potted flower, but also as an open field flower border. It has a wide range of garden uses and is of great ornamental value. This kind of flower is still in the introduction stage in my country. Bellflower is generally propagated by sowing, but its seeds are small, take a long time to germinate, young leaves grow slowly, and the growth cycle is long, and it can bloom only in the next year. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a plant regeneration method of Campanulaceae in view of the deficiencies of the above-mentioned prior art. The method is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/12A01G24/28
CPCA01G31/00A01H4/001A01H4/008A01G24/12A01G24/28
Inventor 赵银萍杜国荣孟长军宋旖李娜
Owner XIAN UNVERSITY OF ARTS & SCI
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