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Molecular target of cervical squamous cell carcinoma and application of molecular target in diagnosis and treatment

A cervical squamous cell carcinoma, horizontal technology, applied in the field of biomedicine, can solve problems such as unsatisfactory results, high metastasis and recurrence rates

Active Publication Date: 2020-07-10
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When cervical cancer further develops to the advanced stage, the effect of surgical resection of cancerous tissue combined with concurrent chemotherapy and radiotherapy is not very satisfactory, and the metastasis and recurrence rates are still high

Method used

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  • Molecular target of cervical squamous cell carcinoma and application of molecular target in diagnosis and treatment
  • Molecular target of cervical squamous cell carcinoma and application of molecular target in diagnosis and treatment
  • Molecular target of cervical squamous cell carcinoma and application of molecular target in diagnosis and treatment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 QPCR detects the expression of RNF167 gene

[0063] 1. Sample collection

[0064] 33 cervical squamous cell carcinoma tissues and corresponding paracancerous tissues were collected. All cases did not receive any immunosuppressant treatment, radiotherapy and chemotherapy before operation.

[0065] 2. RNA extraction

[0066] Tissue total RNA was extracted using TRIZOL method.

[0067] 1) Mince the tissue with scissors, add 1ml Trizol, shake on the shaker for 1min; place at room temperature for 10min to completely decompose the ribosomes.

[0068] 2) Add 200 μl of chloroform (chloroform), close the cap tightly, shake vigorously for 15 seconds, and let stand at room temperature for 10 minutes.

[0069] 3) Centrifuge at 11000 rpm for 15 minutes at 4°C.

[0070] 4) Transfer the water sample layer to a new centrifuge tube, add 500 μl of isopropanol; after inverting to mix well, let stand at room temperature for 10 minutes.

[0071] 5) Centrifuge at 11000 rpm ...

Embodiment 2

[0106] Example 2 Effect of RNF167 on cervical squamous cell carcinoma cells

[0107] 1. Construction of overexpression vector

[0108] According to the RNF167 gene sequence information in NCBI, specific primers were designed and amplified to obtain the corresponding genes. After the gene fragments were recovered by gel, they were connected with the expression vector pGL3 basic and transformed into DH5α competent cells. Positive clones were picked for PCR and sequencing. After identification, the pGL3 basic-RNF167 recombinant plasmid was obtained.

[0109] 2. Cell culture

[0110] Cervical squamous cell carcinoma cells (Hela) were cultured in RPIM-1640 medium containing 10% fetal bovine serum and 1% P / S at 37°C, 5% CO 2 When the cells grew to 80%-90%, they were routinely digested and passaged with 0.25% EDTA-containing trypsin.

[0111] 3. Transfection

[0112] The experiment was divided into 3 groups, namely the control group (Hela), the transfection empty group (transfect...

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Abstract

The invention discloses a molecular target of cervical squamous cell carcinoma and an application of the molecular target in diagnosis and treatment. The inventors find for the first time that expression of RNF167 is down-regulated in patients with the cervical squamous cell carcinoma through QPCR detection, and the RNF167 has higher sensitivity and specificity as a variable, and the result showsthat the RNF167 can be used as the molecular marker for the diagnosis of the cervical squamous cell carcinoma; and through overexpression of the RNF167, the inventors find that the proliferation and invasion ability of cervical cancer cells is significantly affected, and the result shows that the RNF167 can be used as the molecular target for the treatment of the cervical squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a molecular target of cervical squamous cell carcinoma and its application in diagnosis and treatment. Background technique [0002] Cervical cancer (CC) is the most common malignant tumor of the female reproductive system (Chen, W., et al., Cancer statistics in China, 2015. CA Cancer J Clin, 2016.66 (2): p115-32.), its The incidence rate ranks second among gynecological malignancies. According to the World Health Organization (WHO), about 53,000 new cases of cervical cancer are added each year, and as many as 25,000 patients die from cervical cancer (Zhou Qi. Cervical Cancer Diagnosis and Treatment Guidelines (Fourth Edition). Chinese Journal of Practical Gynecology and Obstetrics, 2018, 34(6):613-622.). Due to the popularization of cervical cancer screening in western developed countries, the incidence of cervical cancer has slowly declined, while the situation in developing countries ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61K48/00A61K38/17A61P35/00
CPCA61K38/177A61K45/00A61K48/005A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158G01N33/57411G01N33/57484G01N2333/705
Inventor 彭雪
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN