Compound acellular matrix injection used for treating femur head necrosis, and use method

A technology of acellular matrix and femoral head necrosis, which is applied in bone diseases, medical formulas, drug combinations, etc., can solve the problems of inability to fix mesenchymal stem cells, failure to provide a regenerative microenvironment, and easy loss of stem cells, so as to avoid pioneering surgery risk, speed up the recovery time, and improve the survival rate

Active Publication Date: 2020-07-14
山东隽秀生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The patent (CN201910178248.X) discloses a preparation method of human umbilical cord mesenchymal stem cell injection for lacunar injection in the treatment of ischemic femoral head necrosis. The cartilage differentiation inducer of the injection includes glucose isoflavone, bone Formation protein-2, ascorbic acid, dexamethasone, and sodium β-glycerophosphate, these inducers cannot fix mesenchymal stem cells and cannot provide a regenerative microenvironment, and stem cells are easily lost in the body
The patent (CN201010152461.2) discloses a cell injection for treating bone damage and its preparation method. The gel is mainly poloxamer gel, which belongs to high molecular polymer and is not conducive to renal excretion.
[0008] At present, there are no relevant reports on the composite regenerative materials of acellular matrix fluid, bone marrow mesenchymal stem cells and angelica parasitic fluid to construct a tissue repair microenvironment for the repair of femoral head necrosis tissue

Method used

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  • Compound acellular matrix injection used for treating femur head necrosis, and use method
  • Compound acellular matrix injection used for treating femur head necrosis, and use method
  • Compound acellular matrix injection used for treating femur head necrosis, and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the preparation method of composite acellular matrix injection

[0054] Preparation method of acellular matrix solution: (1) Firstly weigh 500g of porcine Achilles tendon tissue, wash it with PBS and other pretreatments, inactivate the virus and crush it; (2) Add 2L of 10% sodium chloride solution for soaking Shaking table for 2 hours, 30°C / 200rpm; (3) filter, wash the tissue with a large amount of purified water 3 times, 30min each time, shaker for 10min, 30°C / 200rpm; (4) use 2L 3% sodium carbonate Soak the solution for 3 hours to degrease, wash with purified water 3 times: 10min / time, 30℃ / 200rpm; (5) Soak the shaker with 5L 4% trypsin for 3 hours, wash with purified water 3 times: 10min / time, 30℃ / 200rpm; ( 6) Degrade the nucleic acid components in the cells by nuclease, wash thoroughly to remove the detergent to obtain the acellular matrix, and freeze-dry to obtain the acellular matrix powder; (7) prepare pH=2 HCl solution, 10×PBS solution, 1×PBS soluti...

Embodiment 2

[0058] Example 2. Take 1.2 parts of acellular matrix liquid, 1 part of bone marrow mesenchymal stem cell liquid and 0.8 part of Angelica Jisheng injection in the composite acellular matrix injection in Example 1, and mix them according to the proportion during the operation. Fill into a 10ml syringe and compound into an injectable mixture.

Embodiment 3

[0059] Example 3. Take 0.8 parts of acellular matrix liquid, 1 part of bone marrow mesenchymal stem cell liquid and 1.2 parts of Angelica Jisheng injection in the composite acellular matrix injection in Example 1, and mix them according to the proportion during the operation. Fill into a 10ml syringe and compound into an injectable mixture.

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Abstract

The invention discloses a compound acellular matrix injection. The compound acellular matrix injection is taken as a femur head necrosis repairing material for clinical treatment. The compound acellular matrix injection is compounded by biological acellular matrix sol, bone marrow mesenchyml stem cells and radix angelicae sinensis parasitic liquids and is injected into a hip joint position betweenthe head of femur and the acetabulum in a surgery, a micro-environment for tissue repairing can be provided, the bone marrow mesenchyml stem cell is accelerated to carry out proliferation and differentiation to obtain osteoblasts, new vessels and new bones are induced to be formed, and the compound acellular matrix injection also has a function of diminishing inflammation and alleviating pain andcan be used for treating early and medium stage non-traumatic femur head necrosis.

Description

technical field [0001] The invention relates to a preparation method of a composite acellular matrix injection and its therapeutic application in femoral head necrosis, belonging to the field of medical biomaterials and tissue engineering repair. Background technique [0002] The pathogenic factors of non-traumatic femoral head necrosis are mainly divided into two types: chronic alcoholism and abuse of hormone drugs. Alcohol and hormones hinder the blood circulation around the femoral head, causing insufficient blood supply, resulting in necrosis of the femoral head, fracture of trabeculae, collapse of the femoral head, and paralysis of the patient in severe cases. Femoral head necrosis is divided into four stages: , The period is the early stage of subsidence; The period is the period of femoral head collapse, In the early stage, the femoral head collapses and develops into arthritis, which can be treated in the early and middle stages, and artificial hip replacemen...

Claims

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Application Information

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IPC IPC(8): A61K36/232A61K9/08A61K47/46A61P19/08A61L27/38A61L27/36A61L27/50A61L27/54A61L27/58A61K35/28
CPCA61K36/232A61K36/185A61K35/28A61K9/0019A61K9/08A61K47/46A61P19/08A61L27/3834A61L27/3847A61L27/3895A61L27/3637A61L27/3633A61L27/3687A61L27/3691A61L27/365A61L27/50A61L27/54A61L27/58A61L2300/602A61L2430/40A61L2300/412A61L2430/02A61L2300/30A61L2300/41A61L2300/402A61L2400/06A61K2300/00
Inventor 高秀伟高秀岩雷蕾郑红霞
Owner 山东隽秀生物科技股份有限公司
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