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Lactic acid bacteria, and screening and applications thereof

A technology of lactic acid bacteria and Lactobacillus reuteri, applied in bacteria, material separation, biochemical equipment and methods, etc., can solve the problems of less antibiotics and low removal efficiency, and achieve the effect of high degradation rate

Active Publication Date: 2020-07-17
NINGBO UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few studies on the degradation of antibiotics by lactic acid bacteria at home and abroad, and the removal efficiency is low. Therefore, it is of great practical value to explore the application of lactic acid bacteria in the environment.

Method used

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  • Lactic acid bacteria, and screening and applications thereof
  • Lactic acid bacteria, and screening and applications thereof
  • Lactic acid bacteria, and screening and applications thereof

Examples

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Embodiment Construction

[0035] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0036] The screening of the lactic acid bacteria that can degrade ciprofloxacin in this embodiment is carried out by the following method:

[0037] (1) Screening of lactic acid bacteria

[0038] Accurately weigh 10g of the duck intestine sample, cut it into pieces with a sterile scalpel, add it to 90mL of 0.9% normal saline, oscillate and mix well as the concentration gradient is 10 -1 basal culture solution; the basal culture solution was diluted 10 with physiological saline -2 、10 -3 , 10 -4 、10 -5 、10 -6 Five gradients, take 10 -4 、10 -5 、10 -6 Spread 0.1mL of the three dilutions of the culture solution on the MRS agar plate containing 1% calcium carbonate, make three parallels for each dilution, and culture at 37°C for 24 hours;

[0039] Pick a single colony with a calcium fusion circle and further use the MRS agar plate to streak...

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Abstract

The invention relates to lactic acid bacteria, and screening and applications thereof, and relates to a method for testing degradation capacity of screened lactic acid bacteria to ciprofloxacin, and the method is convenient for judging degradation capacity of the lactic acid bacteria. According to the method, through utilization the biodegradation effect of the lactic acid bacteria, lactobacillusreuteri WQ-Y1 which can change the structure of ciprofloxacin through physiological metabolism and further realize harmless treatment of ciprofloxacin on environmental pollution is screened out. According to method for testing degradation capacity of lactic acid bacteria on ciprofloxacin, the lactobacillus reuteri WQ-Y1 can achieve a very high degradation rate of ciprofloxacin under the conditionof short time consumption, and high degradation efficiency of the lactobacillus reuteri WQ-Y1 to ciprofloxacin is reflected; and meanwhile, the method provides a theoretical basis for developing probiotics for degrading ciprofloxacin antibiotic pollution and relieving residues of ciprofloxacin.

Description

technical field [0001] The invention relates to a new lactic acid bacterium, and also relates to the screening of the lactic acid bacterium and the application of the lactic acid bacterium in degrading ciprofloxacin. Background technique [0002] In recent years, the environmental pollution caused by the extensive use of fluoroquinolone antibiotics in medical treatment and livestock breeding has become increasingly serious. Fluoroquinolones are mainly excreted into manure through partial metabolism of animals, and residual fluoroquinolones in animal manure can easily enter the terrestrial environment, and enter the aquatic environment through leaching and direct runoff to spread pharmaceutical pollution. The most common fluoroquinolone antibiotic is ciprofloxacin. [0003] At present, there are mainly physical methods, chemical methods and biological methods to control the spread of antibiotics. Among them, physical methods such as raw material cleaning, resin adsorption, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02G01N30/02G01N30/06G01N30/86C12R1/225
CPCC12N1/20C12N1/02G01N30/02G01N30/06G01N30/8679C12R2001/225C12N1/205Y02A50/20
Inventor 吴振瞿春晓潘道东蔡振东曾小群孙杨赢
Owner NINGBO UNIV
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