Application of SGCE gene as triple negative breast cancer marker

A triple-negative breast cancer and marker technology, applied in the biological field, achieves the effect of simple operation, strong specificity and high sensitivity

Active Publication Date: 2020-07-17
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there is no report on the use of SGCE g...

Method used

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  • Application of SGCE gene as triple negative breast cancer marker
  • Application of SGCE gene as triple negative breast cancer marker
  • Application of SGCE gene as triple negative breast cancer marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 SGCE is highly expressed in breast cancer stem cells

[0026] (1) Screening from single-cell sequencing and finding that SGCE is highly expressed in breast cancer stem cell populations

[0027] Download the raw scRNA-Seq data of published TNBC patients from https: / / github.com / Michorlab / tnbc_scrnaseq website. The Scatter software package was used to perform quality control on single-cell sample data, removing samples with a sequencing depth of less than 1,000 reads, removing samples with a number of detected genes lower than 500 and greater than 10,000, and removing samples with a proportion of mitochondrial-derived reads exceeding 20%. Considering the number of high-quality epithelial cells retained by a single sample after quality control operations, we finally selected tumor epithelial cells from three tumor patients, PT039, PT089, and PT081, for downstream analysis, and grouped the cell populations according to the classification results of the original lit...

Embodiment 2

[0064] Example 2 SGCE is highly expressed in triple-negative breast cancer

[0065] (1) Using TCGA database to analyze the expression level of SGCE in different types of breast cancer

[0066] The result is as figure 2(A) shows: Download the samples of different types of breast cancer patients including Luminal A type, Luminal B type, Her2 and triple-negative breast cancer in the TCGA database, analyze the expression of SGCE in these breast cancer patients and find that SGCE is in The expression level of triple-negative breast cancer is higher than that of other types of breast cancer, and the degree of malignancy of triple-negative breast cancer is higher than that of other three types of breast cancer. The expression level of SGCE is low in breast cancer, and the expression level of SGCE is positively correlated with the malignancy of breast cancer.

[0067] (2) Analyze the expression of SGCE at the protein level in normal breast epithelial cells and triple-negative breas...

Embodiment 3

[0085] Example 3 High expression of SGCE corresponds to poor prognosis of TNBC patients

[0086] Using the kmplot website (http: / / kmplot.com / analysis / ), the relationship between the expression of SGCE and the prognosis and survival rate of breast cancer patients was analyzed ( image 3 ). The high expression of SGCE indicates that the survival period of breast cancer patients is shorter (except Luminal A type), especially the recurrence-free survival (RFS) and disease-free survival (DMFS) prognosis of patients with low SGCE expression in TNBC are good, and in embodiment 2 simultaneously The experimental results also prove that compared with other types of breast cancer, SGCE has the highest expression level in patients with triple-negative breast cancer. These data reveal that the expression level of SGCE is closely related to the malignant process and prognosis of breast cancer, and the high expression of SGCE is closely related to the malignant degree of breast cancer. Posi...

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Abstract

The invention belongs to the technical field of biology and relates to an application of an SGCE gene as a triple negative breast cancer marker. According to the invention, high expression of SGCE intissues or cells of triple negative breast cancer is confirmed through biological information data processing and in vitro and in vivo experimental results, and survival analysis shows that the expression of the SGCE gene is closely related to the prognosis of the triple negative breast cancer, so that the SGCE gene can be used as a marker for diagnosis or prognosis evaluation of the triple negative breast cancer. The invention provides an application of a reagent or a kit for early screening, early diagnosis, early treatment and prognosis evaluation of the triple negative breast cancer, provides a strategy with great prospect for a patient with the triple negative breast cancer to be out of pain, and provides a good tool for basic research and clinical research of breast cancer and othercancers.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the application of SGCE gene, in particular to the application of SGCE gene as a marker of triple-negative breast cancer and its application in the preparation of diagnostic and prognosis evaluation reagents or kits for triple-negative breast cancer. Background technique [0002] Breast cancer is the most common cancer worldwide and one of the leading causes of death in women. Statistics from the American Cancer Society found that in 2019, there were 268,600 new cases of invasive breast cancer and 48,100 new cases of ductal in situ breast cancer, and 41,760 new deaths from breast cancer. As of January 2019, there were more than 38 million breast cancer patients in the United States, including more than 150,000 metastatic breast cancer patients. [0003] According to the results of immunohistochemical staining and high-throughput sequencing, breast cancer can be divided into different t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886C12Q2600/118C12Q2600/158G01N33/57415G01N33/57484
Inventor 焦保卫赵丽娜陈策实邱婷
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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