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Food composition or feed composition containing epoxy group-removed protease and processing method of food composition or feed composition

A technology of feed composition and processing method, applied in the field of diet or feed composition

Pending Publication Date: 2020-07-31
山东农之安生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, no definitive gene or protein has been reported to catalyze the detoxification of trichothecenes by deepoxylation

Method used

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  • Food composition or feed composition containing epoxy group-removed protease and processing method of food composition or feed composition
  • Food composition or feed composition containing epoxy group-removed protease and processing method of food composition or feed composition
  • Food composition or feed composition containing epoxy group-removed protease and processing method of food composition or feed composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] 1. The active polypeptide FTCD catalyzes the removal of epoxy groups from trichothecenes

[0057] 1. Experimental method:

[0058] 1.1 In vitro enzymatic reaction:

[0059] Dissolve DON, 3-DON, 15-ADON, NIV, DAS, HT-2, T-2 toxins (1 mg) in freshly prepared GSH (30.7 mg, 100 μmol) in PBS buffer, and add active peptides, Insulate in a 20°C water bath for 24 hours.

[0060] 1.2 LC-HRMS ( / MS) analysis

[0061] After the in vitro reaction solution was filtered through a 0.22 μm filter membrane, it was transferred to an injection vial for LC-HRMS detection. LC-HRMS( / MS) Analysis Data were analyzed using Xcalibur 2.1.0. The extracted ion chromatograms (EIC) of the toxin and its derivatives were studied using the peak shape, retention time (±0.2 min) and mass (±5 ppm) of the proposed biotransformation products. According to the secondary spectrum and the basic structure of the substance, the neutral loss is analyzed, and the chemical structure is deduced.

[0062] 2. Expe...

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PUM

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Abstract

The invention discloses a food composition or a feed composition containing epoxy group-removed protease and a processing method of the food composition or thefeed composition. The food composition orthe feed composition comprises the epoxy group-removed protease. The protease can enable trichothecene toxin to catalytically react glutathione at a temperature of 15-35 DEG C to produce a glutathione derivative. According to the method, toxin-polluted cereal products are subjected to in-vitro enzyme catalysis treatment through the epoxy group-removed protease, so that the detoxification requirements of the feed industry, the food industry and the like on trichothecene toxin are met. The invention fills up the blank that no clear protein can be used for detoxifying through epoxy group-removedcatalyzing on the trichothecene toxin at present, and has a wide application prospect in trichothecene toxin detoxification related to foods or feeds.

Description

technical field [0001] The invention relates to the field of diet or feed composition, in particular to a diet or feed composition containing a protease with catalytic activity for removing epoxy groups and a processing method thereof. Background technique [0002] The basic chemical structure of trichothecenes is a sesquiterpene, which is also called 12,13-epoxy trichothecenes because of the formation of epoxy groups on the 12th and 13th carbons compound. Since the 1970s, researchers have found that the epoxy group of trichothecenes is the main source of toxicity. Trichothecenes can be found in large quantities in plants or grains infected by Fusarium, a worldwide distribution of fungi that not only survive winter and summer in soil, but also invade It infects a variety of plants, causing various diseases such as root rot, stem rot, stem base rot, flower rot and ear rot, etc. There are more than 100 species of host plants, infecting the vascular system of host plants and ...

Claims

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Application Information

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IPC IPC(8): A23L5/20A23L2/84A23L2/70A23C7/04C12N9/50
CPCA23L5/25A23L5/27A23L2/84A23L2/70A23C7/04C12N9/50A23V2002/00A23V2250/31
Inventor 不公告发明人
Owner 山东农之安生物科技有限公司