Simulated gall bladder wall synthesis material, and preparation method and application thereof and simulated gall bladder wall

Abstract

The invention relates to a preparation method of a simulated gall bladder wall synthesis material, the simulated gall bladder wall synthesis material and a simulated gall bladder wall, and an application of the simulated gall bladder wall synthesis material to preparation of the simulated gall bladder wall. The preparation method comprises the steps of A, dissolving chitosan in acidic liquid, to prepare a chitosan solution of which the chitosan mass concentration is 0.5%-12%; B, based on the chitosan solution, preparing a chitosan bracket; C, adding adipose-derived stem cells cultured througha first culture medium to hydrogel to obtain a mixture having preset cell density, and casting the mixture to the chitosan bracket; and D, casting a silk fibroin solution onto the mixture cast to thechitosan bracket, to form a composite bracket, performing a cross-linking reaction on the composite bracket and a CaCl2 solution of which the mass concentration is 0.5%-10% for 5-60 minutes, and performing placing in a second culture medium to prepare the simulated gall bladder wall synthesis material. The simulated gall bladder wall synthesis material has notable practicality and safety, and is favorable for improvement of human health medical quality.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a preparation method of a synthetic material for a simulated gallbladder wall, a synthetic material for a simulated gallbladder wall, a simulated gallbladder wall, and an application of the synthetic material for a simulated gallbladder wall in preparing a simulated gallbladder wall. Background technique [0002] Currently, patients with gallbladder disease in China have reached 20% of the population. Common gallbladder diseases include gallstones and gallbladder polyps, which are often accompanied by cholecystitis. The incidence of gallbladder stones is 10%-12%, of which 30%-50% are caused by abnormal gallbladder morphology such as gallbladder wall folding, gallbladder wall ring thickening with stenosis, and gallbladder wall septation. In this regard, cholecystectomy is the most commonly used treatment at present, but many complications such as indigestion, abdomin...

AI Technical Summary

Problems solved by technology

If only the polyp is removed without treating the gallbladder wall at the pedicle of the polyp, the possibility of canceration at the pedicle of the residual polyp cannot be completely eliminated

Similarly, if the diseased gallbladder wall is removed and the remaining gallbladder is too small, there is no point in gallbladder preservation

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