Preparation method of bletilla striata polysaccharide

The technology of Bletilla striata polysaccharide and Bletilla striata, which is applied in the field of medicine and chemical industry, can solve the problems of high irritation and can not meet the requirements of beauty products, and achieve the effects of reducing irritation, meeting the needs of mildness and improving the dissolution rate.

Inactive Publication Date: 2020-07-31
ZHONGSHAN FLASHLIGHT POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the rise of modern people's skin sensitivity rate, the pursuit of effectiveness and mildness of beauty products has also increased. Organic solvent extracts are often too irritating, and high-temperature extraction has a greater impact on the activity of heat-sensitive active ingredients. These Traditional extraction methods can no longer meet the requirements for people's beauty products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of bletilla striata polysaccharide, characterized in that: comprising the following steps:

[0025] (1) Pulverizing: 1 kg of Bletilla striata tuber is pulverized with a pre-installed universal pulverizer of 80 mesh screens to obtain Bletilla striata coarse powder;

[0026] (2) Ultrasonic treatment: take the coarse powder of Bletilla striata prepared in step (1) and add 5 times the volume of water, then ultrasonically extract at 40°C for 60 minutes, repeat the extraction 3 times, and combine the extracts to obtain the ultrasonic treatment solution;

[0027] (3) Enzymolysis treatment: add 2500U / g cellulase to the ultrasonic treatment solution prepared in step (2), enzymolyze it at 55°C for 2h, centrifuge at 4000r / min for 30min, and collect the supernatant to obtain crude Bletilla striata. polysaccharide extract;

[0028] (4) microfiltration concentration: the bletilla striata polysaccharide extract obtained in step (3) is subjected to tangential flow...

Embodiment 2

[0030] A preparation method of bletilla striata polysaccharide, characterized in that: comprising the following steps:

[0031] (1) Pulverize: 5kg bletilla striata tuber is pulverized with the universal pulverizer of preloaded 80 mesh sieves, obtains bletilla striata coarse powder;

[0032] (2) Ultrasonic treatment: take the coarse powder of Bletilla striata prepared in step (1) and add 10 times the volume of water, then ultrasonically extract at 35°C for 1 hour, repeat the extraction 3 times, and combine the extracts to obtain the ultrasonic treatment solution;

[0033] (3) Enzymolysis treatment: add 2500U / g cellulase to the ultrasonic treatment solution prepared in step (2), enzymolyze it at 55°C for 2h, centrifuge at 4000r / min for 30min, and collect the supernatant to obtain crude Bletilla striata. polysaccharide extract;

[0034] (4) microfiltration concentration: the bletilla striata polysaccharide extract that step (3) makes is filtered through the microfiltration filte...

Embodiment 3

[0036] A preparation method of bletilla striata polysaccharide, characterized in that: comprising the following steps:

[0037] (1) Pulverizing: 1 kg of Bletilla striata tuber is pulverized with a universal pulverizer of pre-installed 60 mesh sieve, to obtain Bletilla striata coarse powder;

[0038] (2) Ultrasonic treatment: take the Bletilla striata coarse powder prepared in step (1) and add 5 times the volume of water, then ultrasonically extract at 10°C for 10 minutes, repeat the extraction twice, and combine the extracts to obtain the ultrasonic treatment solution;

[0039] (3) Enzymolysis treatment: add 2000U / g cellulase to the ultrasonic treatment solution prepared in step (2), enzymolyze it at 35°C for 1 hour, centrifuge at 4000r / min for 30 minutes, collect the supernatant to obtain crude Bletilla striata. polysaccharide extract;

[0040] (4) microfiltration concentration: the bletilla striata polysaccharide extract obtained in step (3) is subjected to tangential flow ...

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Abstract

The invention discloses a preparation method of bletilla striata polysaccharide, which comprises the following steps: (1) pulverizing: pulverizing bletilla striata tubers to obtain bletilla striata coarse powder; (2) ultrasonic treatment: adding a proper amount of water into the bletilla striata coarse powder prepared in the step (1), and carrying out ultrasonic extraction to obtain an ultrasonictreatment liquid; (3) enzymolysis treatment: adding cellulase into the ultrasonic treatment liquid prepared in the step (2) for treatment, and centrifuging after treatment to obtain a bletilla striatapolysaccharide extract liquid; and (4) microfiltration and concentration: carrying out tangential flow filtration on the bletilla striata polysaccharide extract liquid prepared in the step (3) by using a ceramic membrane filter, and collecting microfiltration trapped fluid with required concentration to obtain colloidal liquid bletilla striata polysaccharide. The preparation method can be used for efficiently extracting bletilla striata polysaccharide in an environment-friendly manner and also can be used for effectively improving the extraction purity.

Description

technical field [0001] The invention relates to the technical field of medicine and chemical industry, in particular to a preparation method of bletilla striata polysaccharide. Background technique [0002] Bletilla striata (Thunb.) Reichb.f) is a plant of the genus Bletilla striata in the family Orchidaceae, also known as Bletilla striata, Xuerumo, etc. Bletilla striata contains a large amount of water-soluble polysaccharides, and its chemical composition is glucomannan, which is its main functional component. Bletilla striata polysaccharide has the effect of oral anti-oxidation and external application to promote the migration of keratinocytes in medicine. It can be used as an arterial embolism for the treatment of liver cancer, and it can also be used for the treatment of ulcerative colitis, hemostasis in surgery, acute upper gastrointestinal bleeding and promotion. Skin wound healing, etc.; in the food industry, it can be used as an important raw material for natural fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/0087
Inventor 戴洁王琼周子明
Owner ZHONGSHAN FLASHLIGHT POLYTECHNIC
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