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Novel coronavirus detection kit and preparation method and detection method thereof

A detection kit and coronavirus technology, which is applied in the field of novel coronavirus detection kits and its preparation, can solve the problems of low detection efficiency and high detection requirements, and achieve the effect of simple operation, efficient and convenient detection

Pending Publication Date: 2020-07-31
青岛爱博检测科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a novel coronavirus detection kit and its preparation method and detection method, to solve the defects of high detection requirements and low detection efficiency in existing novel coronavirus detection

Method used

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  • Novel coronavirus detection kit and preparation method and detection method thereof
  • Novel coronavirus detection kit and preparation method and detection method thereof
  • Novel coronavirus detection kit and preparation method and detection method thereof

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Embodiment Construction

[0025] The specific embodiments of the present invention will be described in detail below, and it should be understood that the specific embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0026] Such as figure 1 As shown, the novel coronavirus detection kit of the present invention includes a handle area 1, which is made of absorbent paper

[0027] Prepared; test area 2, which uses nitrocellulose membrane as a carrier, and the nitrocellulose membrane is respectively coated with anti-rabbit IgG polyclonal antibody, anti-human IgG antibody, and anti-human IgM antibody from top to bottom; gold pad: it includes Polyester film, the polyester film is coated with a gold label conjugate formed by combining colloidal gold and the recombinant antigen of the new coronavirus; or, the gold pad includes a polyester film, and the polyester film is coated with a gold label formed by combining colloi...

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Abstract

The invention discloses a novel coronavirus detection kit and a preparation method and a detection method thereof. The detection kit comprises a handle area, a test area, a gold pad, a sample pad anda blood filtering membrane, wherein the handle area is prepared from absorbent paper; the test area takes a nitrocellulose membrane as a carrier, and the nitrocellulose membrane is respectively coatedwith an anti-rabbit IgG polyclonal antibody, an anti-human IgG antibody and an anti-human IgM antibody from top to bottom; the gold pad comprises a polyester film, and the polyester film is coated with a gold-labeled conjugate formed by combining colloidal gold and a novel coronavirus recombinant antigen; the sample pad comprises glass fibers soaked in a buffer solution; and the blood filtering membrane is attached to the sample pad. The novel coronavirus detection kit adopts an immune colloidal gold chromatography technology, and colloidal gold is used as a tracing marker to be applied to antigens and antibodies in a novel immunolabeling technology. Novel coronavirus IgG / IgM antibodies in human serum, plasma and whole blood samples are qualitatively detected in vitro, and the detection process is simple to operate.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a novel coronavirus detection kit, a preparation method and a detection method thereof. Background technique [0002] Accurate detection is an important part of the prevention and control of the new crown pneumonia epidemic. At present, there are two main types of new crown virus detection, one is nucleic acid detection, and the other is immune detection. Nucleic acid detection is mainly PCR. By extracting viral RNA, reverse transcription, and amplifying the conserved sequence of the virus, the fluorescent protein in the amplification system will combine with the amplified double-stranded DNA sequence to emit fluorescence. Judging by the detected fluorescence value Whether virus is present and quantified. This kind of test has relatively high requirements, and requires professional instruments and molecular testing laboratories as well as professional operato...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56983G01N33/558G01N33/54313G01N2333/165
Inventor 姚智博
Owner 青岛爱博检测科技有限公司
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