A method for automatic preprocessing of cryo-electron microscopy single particle analysis data

Abstract

The invention discloses a method for automatic preprocessing of cryo-electron microscope single-particle analysis data. The method includes the following steps: first, user login is performed, and identity verification is performed by means of an identity verification system; the identity verification system is automatically bound to the group account of the electron microscope platform The present invention needs to first verify the security of the device when the user logs in. The verification method is to enter the login information through the data entry unit, and through special processing of the login information, a set of values ​​can be obtained; at the same time, the first glimpse unit also There are the same built-in rules for processing the login information to obtain a set of standard values, compare the standard values ​​with the values, and only when they are consistent will a device verification signal be generated; after completing the verification of the device, it will be based on the specific The processed selected feature value is selected for further verification. There are two preset verification methods, and one of the two is selected according to the selected feature value; thereby completing the identity verification.

Description

technical field [0001] The invention belongs to the field of data processing, and relates to a cryo-electron microscope data processing technology, in particular to a method for automatic preprocessing of cryo-electron microscope single particle analysis data. Background technique [0002] With the rapid development of low-temperature transmission electron microscopy (cryo-electron microscopy for short), a major technology in structural biology, domestic scientific research institutions have built cryo-electron microscopy platforms and supporting electron microscopy data computing platforms. For scientific users, collecting a set of high-quality electron microscopy data is crucial. [0003] During the data acquisition process, the original data belongs to the unprocessed movie-stack, and cannot be directly viewed and evaluated for data quality. Users need to manually perform more than two steps of preprocessing, including motion-correction for offset correction, so that the ...

AI Technical Summary

Problems solved by technology

At present, all major electron microscopy platforms use scripts to call preprocessing software to achieve preprocessing when collecting data. If parameter modification is required, it needs to be realized by modifying the script. There is no friendly interface to realize parameter modification.

At the same time, the loading of the preprocessing results display is slow, the display information is limited and statistics cannot be done

Preprocessing through script calls will cause the preprocessed data to be unable to be de-embedded with the mainstream software RELION, which will affect the subsequent resolution improvement steps such as CTFrefine and BayesianPolishing, and the original data can only be preprocessed with RELION software. The processing completes the whole process steps; and the current processing of cryo-electron microscope data does not have privacy, and there is no effective verification method for the user's identity. The specific identity of the user can be verified according to the verification method to avoid data being embezzled. Ensure the reasonable use of user accounts; in order to realize this idea, a solution is now provided

Images