Preparation method of benserazide hydrochloride impurity
A technology of benserazide hydrochloride and impurities, which is applied in the field of medicine and can solve problems such as low efficiency, waste, and poor stability
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Embodiment 1
[0020] Add 9.6g of benserazide hydrochloride and 30ml of water into a 100ml three-necked flask, heat to 20-30°C and stir for 16-20 hours, then concentrate to remove water. Add 20mL of methanol, lower the temperature to 0-10°C, add 80mL of ethanol dropwise, precipitate a solid and keep stirring, then suction filter to obtain the crude product. Add 20mL of methanol and 10mL of water to the crude product at 10-20°C and stir to dissolve, then add dropwise 30mL of ethanol to stir and crystallize, filter and dry to obtain Pharmacopoeia Impurity B of Benserazide Hydrochloride, 4.1g, yield 55.5%. 1 H NMR (400 MHz, DMSO-d 6 )δ9.83(s,1H),8.76(s,2H),8.16(s,4H),6.35(d,J=8.3Hz,2H),6.14(d,J=8.2Hz,2H),5.39( t,J=4.9Hz,1H),4.30(d,J=4.1Hz,1H),3.81–3.64(m,1H),3.73(d,4H),3.51–3.33(m,2H).Mass:396.1 [M+H].
Embodiment 2
[0022] Add 2.0g of benserazide hydrochloride and 6ml of water into a 100ml three-necked flask, heat to 30-40°C and stir for 6-8 hours, then concentrate to remove water. Add 30mL of methanol, lower the temperature to 0-10°C, precipitate a solid, heat it and stir it, then filter it with suction to obtain the crude product. Add 20mL of methanol and 10mL of water to the crude product at 10-20°C and stir to dissolve, then dropwise add 30mL of methanol to stir and crystallize, filter and dry to obtain Pharmacopoeia impurity B of benserazide hydrochloride, 0.82g, yield 53.2%. 1 H NMR (400MHz, DMSO-d 6 )δ9.83 (s, 1H), 8.76 (s, 2H), 8.16 (s, 4H), 6.35 (d, J = 8.3Hz, 2H), 6.14 (d, J = 8.2Hz, 2H), 5.39 ( t,J=4.9Hz,1H),4.30(d,J=4.1Hz,1H),3.81–3.64(m,1H),3.73(d,4H),3.51–3.33(m,2H).Mass:396.1 [M+H].
Embodiment 3
[0024] Add 2.0g of benserazide hydrochloride and 6ml of water into a 100ml three-necked flask, heat to 30-40°C and stir for 6-8 hours, then concentrate to remove water. Add 30mL of ethanol, lower the temperature to 0-10°C, precipitate a solid, heat it and stir it, then filter it with suction to obtain the crude product. Add 20mL of ethanol and 10mL of water to the crude product at 10-20°C and stir to dissolve, then add dropwise 30mL of ethanol to stir and crystallize, filter and dry to obtain Pharmacopoeia impurity B of benserazide hydrochloride, 0.88g, yield 57.1%. 1 H NMR (400MHz, DMSO-d 6 )δ9.83 (s, 1H), 8.76 (s, 2H), 8.16 (s, 4H), 6.35 (d, J = 8.3Hz, 2H), 6.14 (d, J = 8.2Hz, 2H), 5.39 ( t,J=4.9Hz,1H),4.30(d,J=4.1Hz,1H),3.81–3.64(m,1H),3.73(d,4H),3.51–3.33(m,2H).Mass:396.1 [M+H].
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