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Digital PCR droplet generation device and generation method

A droplet generation, digital technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as unfavorable observation and analysis, low detection efficiency, occupation of instrument resources, etc., to achieve savings The overall operation time, the implementation method is simple, and the effect of improving the detection efficiency

Pending Publication Date: 2020-08-04
BEIJING ZHIYU BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, routine blood tests, cytology, pathology, and immunology are all developing in the direction of automation, integration, and standardization. Many difficult technical problems
As far as the micro-droplet observation of the digital PCR detector is concerned, if the droplet array microscopic imaging method is used, the generated droplets need to be transferred and tiled. If the droplets overlap with each other, it is not conducive to observation and analysis
The traditional way to promote droplet transfer and tiling is to use some peripheral drive components or drive structures such as negative pressure pumps to generate negative or positive pressure. The structure and control methods are relatively complex and require additional instrument resources. , but also requires additional operating time, and the detection efficiency is relatively low

Method used

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  • Digital PCR droplet generation device and generation method
  • Digital PCR droplet generation device and generation method
  • Digital PCR droplet generation device and generation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] see Figure 1 to Figure 4 As shown, a digital PCR droplet generation device includes a chip body 1, which has a droplet generation chamber 4 and a droplet storage chamber 6 communicated with the droplet generation chamber 4, and the droplet is generated in the liquid The droplet generation chamber 4 is completed, and the generated droplets are transferred to the droplet storage chamber 6 to be tiled and detected. The droplet generating device also includes a liquid absorbing liquid 9 with a liquid absorbing function. The liquid absorbing liquid 9 cooperates with the chip body 1 and is used to drive the liquid droplet to transfer from the droplet generating chamber 4 to the droplet storage chamber 6 .

[0063] Specifically, see Figure 1 to Figure 4 As shown, the chip body 1 also has a liquid discharge chamber 7 communicated with the droplet storage chamber 6, and a liquid absorbing liquid 9 can be inserted in the liquid discharge chamber 7 for absorbing liquid and prom...

Embodiment 2

[0082] see Figure 5 to Figure 7 The main difference between the shown digital PCR droplet generation device and Embodiment 1 is that the lower part of the main part 9a of the liquid absorbing liquid 9 also has a root part 9d extending downward, and the cross-sectional size of the root part 9d is smaller than that of the main part 9a The cross-sectional size is to form a section tightening structure at the bottom of the liquid absorption 9, so that the liquid can be sucked out from the liquid discharge port 8, and the liquid droplets are transferred to the liquid drop storage chamber 6. The cross-sectional area of ​​the root 9d can determine the magnitude of the negative pressure generated, and further determine the velocity of the liquid flow. Preferably, the cross-sectional area of ​​the root portion 9d is preferably 0.25-1mm 2 , the length of which is preferably 1-8mm, which can generate a more stable negative pressure, so that the droplets can be sucked into the droplet s...

Embodiment 3

[0089] see Figure 9 to Figure 10 As shown, it shows another structural form of the chip body 1 , which has a single droplet storage chamber 6 , and correspondingly only has a set of liquid inlet pipes 2 and liquid outlet pipes 3 . The setting of the droplet storage chamber 6 adopts a polygonal shape, and the liquid inlet 5 and the liquid outlet 8 are directly connected to the droplet storage chamber 6 on the diagonal corners of the polygon of the droplet storage chamber 6, and the droplets are in the droplet generation chamber 4 After the generation is completed, the suction liquid 9 is inserted into the liquid discharge chamber 7, the oil phase in the droplet storage chamber 6 can be absorbed, and the droplets are transferred from the droplet generation chamber 4 to the droplet storage chamber 6 through the liquid inlet 5 Inside.

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Abstract

The invention discloses digital PCR droplet generation device and droplet generation method. The liquid drop generating device comprises a chip body, wherein the chip body is provided with a liquid drop generating cavity and a liquid drop storage cavity communicated with the liquid drop generating cavity, the liquid drop generating device further comprises a liquid absorbing body with a liquid absorbing function, and the liquid absorbing body is used in cooperation with the chip body and used for driving liquid drops to move to the liquid drop storage cavity from the liquid drop generating cavity. After liquid drops begin to be generated in the liquid drop generation cavity or the liquid drops are generated, the liquid is sucked through the liquid suction body with the liquid suction function, the liquid drops in the liquid drop generation cavity are promoted to be gradually transferred into the liquid drop storage cavity, and therefore the spreading process in the liquid drop storagecavity is achieved step by step. Different from the traditional scheme that a negative pressure device is adopted to promote liquid drops to enter and finish spreading in a liquid drop storage cavity,the implementation mode is simple and effective, instrument resources are not occupied, the cost is remarkably reduced, and the detection efficiency is greatly improved.

Description

technical field [0001] The invention relates to a digital PCR droplet generating device and a generating method. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical laboratory urgently needs fast and accurate detection methods, and molecular detection has unique advantages. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology. Centers, technical service centers, third-party testing agencies, and microbial rapid testing markets. [0004] As an important technical means of molecular detection, PCR can qualitatively and quantitatively detect target accounting molecules. Under the background of increasing application requirements such as low-abundance detection and rare mutation detection, digital PCR is an absolute quantitative technology for nucleic acid molecules. Distribute...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/34B01L3/00
CPCB01L3/502784B01L2200/0626B01L2200/12C12Q1/686C12Q2563/159
Inventor 李昂周阳
Owner BEIJING ZHIYU BIOTECH LTD