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Method for quantitatively evaluating fungal zoospore or bacterial chemotaxis

A technology for quantitative evaluation of zoospores, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of high cost, difficult quantitative evaluation of chemotaxis, cumbersome operation steps, etc., to prevent bubble formation, Quantitatively evaluate the effect of objective and accurate results

Pending Publication Date: 2020-08-04
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemotaxis devices reported in the current literature have major defects in accurate counting. Many chemotaxis devices can only provide an estimated value, and cannot accurately count the chemotaxis process of microorganisms.
In addition, some chemotaxis devices are more complex in design and need to be used with a spectrophotometer or dark field microscope. The operation steps are cumbersome and the cost is high.
All of these have made it difficult to quantitatively evaluate chemotaxis with existing technologies.

Method used

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  • Method for quantitatively evaluating fungal zoospore or bacterial chemotaxis
  • Method for quantitatively evaluating fungal zoospore or bacterial chemotaxis
  • Method for quantitatively evaluating fungal zoospore or bacterial chemotaxis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 Chemotaxis of zoospores of Phytophthora capsici to ethanol

[0051]1. Build a zoospore chemotaxis device. Two capillaries with an inner diameter of 0.3 mm and a length of 6.0 cm, and two capillaries with a length of 2.0 cm and four capillaries with a length of 0.95 cm and an inner diameter of 0.9 mm were prepared. Fix it on a glass slide with 2-cyanoacrylate ethyl glue; prepare ultrapure water containing 0.2% Tween-20, add ethanol to it, and prepare a 20% ethanol solution as a treatment group, with Ultrapure water containing 0.2% Tween-20 was used as the control group. Use a capillary with an inner diameter of 0.3mm to absorb the treatment solution and the control solution respectively. The liquid must fill the entire capillary, wipe off the excess liquid on the outer wall of the capillary with toilet paper, cut off a 2.0cm capillary, and place the capillary containing the treatment and control solutions on the left and right sides respectively Raise the ...

Embodiment 2

[0061] Embodiment 2 The chemotaxis of Phytophthora sojae zoospores to cinnamic acid standard solution

[0062] 1. Build a zoospore chemotaxis device. Prepare 2 capillaries with an inner diameter of 0.3mm and a length of 6.0cm, and 2 capillaries with a length of 2.0cm and 4 capillaries with a length of 0.95cm and an inner diameter of 0.9mm, and fix them on the glass slide with 2-cyanoacrylate ethyl ester glue Prepare concentration as the cinnamic acid standard solution of 1ppm as the treatment group, with the ethanol solution containing the same concentration as the control group in the treatment. Use a capillary with an inner diameter of 0.3mm to absorb the treatment solution and the control solution respectively. The liquid must fill the entire capillary, wipe off the excess liquid on the outer wall of the capillary with toilet paper, cut off a 2.0cm capillary, and place the capillary containing the treatment and control solutions on the left and right sides respectively Rai...

Embodiment 3

[0069] Embodiment 3 Bacillus amyloliquefaciens chemotaxis to dibutyl phthalate

[0070] 1. Build a bacterial chemotaxis device. Prepare 2 capillaries with an inner diameter of 0.9mm and a length of 6.0cm, and 2 capillaries with a length of 2.0cm and 4 capillaries with a length of 0.95cm and an inner diameter of 0.9mm, and fix them on the glass slide with 2-cyanoacrylate ethyl ester glue ; Prepare a solution of dibutyl phthalate and 1% ethanol at a concentration of 100ppm as a treatment group, and a solution containing 1% ethanol as a control group. Use a capillary with an inner diameter of 0.9mm to absorb the treatment solution and the control solution respectively. The liquid must fill the entire capillary, wipe off the excess liquid on the outer wall of the capillary with toilet paper, cut off a 2.0cm capillary, and place the capillary containing the treatment and control solutions on the left and right sides respectively Raise the top of the capillary so that an angle of 2...

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Abstract

The invention belongs to a chemotaxis evaluation method, and particularly discloses a method for quantitatively evaluating fungal zoospore or bacterial chemotaxis. According to the method, a capillarychemotaxis device is built, a test solution and a control solution are placed in the same sample adding tank at the same time, and the chemotaxis phenomenon is quantitatively evaluated by comparing the quantity of zoospore or bacteria aggregated in two capillaries added with different chemical substances and calculating according to a chemotaxis index calculation formula. The tail end of the capillary tube is closed, so that the treating fluid in the capillary tube is stabilized in the capillary tube, and convection between the treating fluid and spore suspension or bacterium suspension is reduced. According to the method, the included angle between the capillary tube and the glass slide is 2-3 degrees, so that on one hand, bubbles are prevented from being formed at the opening of the capillary tube, and on the other hand, zoospore is prevented from passively entering the capillary tube along with liquid flowing, and the quantitative evaluation result of the method can be more objective and accurate.

Description

technical field [0001] The invention belongs to a method for evaluating chemotaxis, in particular to a method for quantitatively evaluating the chemotaxis of zoospores or bacteria. Background technique [0002] Chemotaxis is the behavior that organisms sense the chemical substances in the environment and approach these chemical substances through their own movement. [0003] Many microorganisms rely on chemotaxis to contact the host by sensing the chemical signal released by the host, and finally invade the host. Many pathogenic bacteria and bacteria have specific chemotactic substances. At present, the research on chemokines has become a hot spot at home and abroad. Therefore, the construction of a microbial chemotaxis reaction observation device has become the basis for the study of chemotaxis reactions. However, the chemotaxis devices reported in the literature have major defects in accurate counting. Many chemotaxis devices can only provide an estimated value, and canno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 刘屹湘吴家庆朱书生杨敏梅馨月黄惠川杜飞韩光煜张贺何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY