Dilution method of deep sea in-situ gradient circulation dilution device
A dilution device and a gradient dilution technology, applied in the direction of measuring devices, test sample preparation, sampling, etc., can solve the problems of loss of scientific data results, large instrument volume and power consumption, inconvenient on-site operations, etc., to achieve simple structure and low cost. Low, high precision effect
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Embodiment 1
[0031] see figure 1 , which provides a structural schematic diagram of a deep-sea in-situ gradient circulation dilution device for the present invention, including: a dilution chamber 110, a pump 120, a first four-way distribution valve 130, a second four-way distribution valve 140, a first two-way distribution valve 150. A plurality of parallel gradient dilution channels 160, a first reagent bag 170 and a second reagent bag 180, any one of the plurality of parallel gradient dilution channels 160 is formed by connecting several two-way distribution valves, and the first The reagent bag 170 stores the diluent, and the second reagent bag 180 stores the standard solution.
[0032] The structure and connection relationship of each component will be described in detail below.
[0033] Specifically, both the first reagent bag 170 and the second reagent bag 180 are connected to two ports of the first four-way distribution valve 130, and the other port of the first four-way distribut...
Embodiment 2
[0048] see figure 2 , is a flow chart of working steps of a deep-sea in-situ gradient circulating dilution device provided in Example 2 of the present invention, comprising the following steps:
[0049] Step S110: Fill the pipeline with the diluent stored in the first reagent bag, and make the pump 120, the first four-way distributing valve 130, the second four-way distributing valve 140, the first two-way distributing valve 150, and more All the parallel gradient dilution channels 160 are closed;
[0050] Specifically, open the first reagent bag, fill the pipeline with the diluent stored in the first reagent bag, so that all the pipelines and pump valve cavities are filled with the diluted base liquid, and all the pump valves are in the state of Disabled.
[0051] Step S120: Open the first four-way distribution valve and the second four-way distribution valve, open the two-way distribution valve in one of the parallel gradient dilution channels, start the pump, draw excess...
Embodiment
[0070] see again figure 1 , taking the dilution of the 10mm-100mm standard solution to 2nm-20nm as an example, two dilutions are required. The first time passes through a 1ml quantitative loop, dilutes 100 times, and dilutes to 100nm-1000nm; the second time passes through a 2ml quantitative loop, dilutes to 2nm-20nm.
[0071] Preparation before dilution:
[0072] a) All pipelines and pump valve cavities are filled with diluted bottom liquid;
[0073] b) Empty the 100ml solution cavity, and move the piston to the leftmost end;
[0074] c) All pump valves are closed before working.
[0075] First dilution workflow (dilution 100 times):
[0076] a) Port C of No. 1 four-way distribution valve is opened, port A of No. 2 four-way distribution valve is opened, port B of No. 1 two-way distribution valve is opened, port A of No. 2 two-way distribution valve is opened, and the plunger pump is started to extract excess Standard solution (at least 4 times the volume of the flow path)...
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