Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Genetic marker related to goat cashmere fiber diameter and application thereof

A fiber diameter, cashmere technology, applied in the field of genetic markers

Active Publication Date: 2020-08-18
GANSU AGRI UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The KRTAP2 gene family has been identified in the human genome, which belongs to HS-KAPs and consists of five members, KRTAP2-1, KRTAP2-2, KRTAP2-3, KRTAP2-4 and KRTAP2-5P (pseudogene). Studying the goat KRTAP2 gene family

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Genetic marker related to goat cashmere fiber diameter and application thereof
  • Genetic marker related to goat cashmere fiber diameter and application thereof
  • Genetic marker related to goat cashmere fiber diameter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1 Materials and methods

[0048] 1.1 Test material

[0049] In Huanxian Yusheng Cashmere Goat Breeding Co., Ltd., Qingyang City, Gansu Province, 8 unrelated Longdong Cashmere Goat rams were used as male parents, and Longdong Cashmere Goat ewes were bred by artificial insemination technology. 249 normally developed Longdong cashmere goats were randomly selected among the offspring, and the cashmere production and cashmere height were measured on the spot when they were one year old. Cashmere samples were collected at the midline of the back and brought back to the laboratory to determine the mean fiber diameter (Mean Fiber Diameter, MFD) of cashmere. For the above 249 individuals, 8 mL of blood was collected from the jugular vein of each sheep, anticoagulated with citric acid dextrose (ACD), stored at -20°C, and genomic DNA was extracted by phenol-chloroform extraction.

[0050] 1.2 Goat genome sequence analysis

[0051] According to the coding region sequence of the ...

Embodiment 2

[0105] The cashmere fiber diameter detection PCR-SSCR kit of Longdong cashmere goat of the present invention comprises:

[0106] 1. PCR reaction system

[0107] The 20 μL PCR reaction system is: 10 μL Taq premixed enzyme (Shanghai Huada Gene Technology Co., Ltd.), ddH 2 O 7.6 μL, 0.25 μmol L -1 0.8 μL each of the upstream and downstream primers, 50ng·μL -1 0.8 μl of genomic DNA template.

[0108] Among them, the upstream primer: 5'-TCTGGGGATCATCATCTCCT-3'

[0109] Downstream primer: 5'-GGGAGGTCCTGAAGGTGCGG-3'.

[0110] 2. SSCP Loading Denaturation Buffer

[0111] Including 98% deionized formamide, 10mmol·L -1 EDTA, 0.025% bromophenol blue and 0.025% xylene cyanol.

[0112] 3. Standard sample

[0113] Standard sample A: its nucleotide sequence is SEQ ID No.1 in the sequence table;

[0114] Standard sample B: its nucleotide sequence is SEQ ID No.2 in the sequence listing.

[0115] Standard sample C (also used as a positive control): its nucleotide sequence is SEQ ID No...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a genetic marker related to goat cashmere fiber diameter, the genetic marker is located on a KRTAP2-1 gene and has three dominant alleles A, B and C, and the nucleotide sequences of the alleles A, B and C are respectively SEQ ID No. 1-SEQ ID No. 3 in a sequence table; the cashmere fiber diameter of the individual containing the allele C is minimum; and the cashmere fiber diameter of the individual without the allele C is the largest. The genetic marker disclosed by the invention can be used for identifying the cashmere fiber diameter of the Longdong cashmere goats, and can be used for selecting the Longdong cashmere goats with small cashmere fiber diameters and eliminating the Longdong cashmere goats with large cashmere fiber diameters.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, in particular to a genetic marker related to the fiber diameter of goat cashmere and its application. Background technique [0002] Cashmere fiber is produced by secondary hair follicles. It has the advantages of thin and soft, light and warm, smooth and plump suede, good elasticity and moisture absorption, unique style and comfortable wearing. Its price is much higher than that of sheep wool and mohair. Known as "soft gold" and "fiber diamond". Among the economic properties of cashmere, cashmere yield and average fiber diameter are the two most important properties, which directly determine the economic value of cashmere. [0003] Keratins (Ks) and keratin-associated proteins (KAPs; their coding genes are identified as KRTAPs) are the main structural proteins of cashmere fibers, accounting for about 90% of its total weight. The former form keratin intermediate filaments (keratin inte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02A50/30
Inventor 王继卿孙凯罗玉柱刘秀李少斌胡江
Owner GANSU AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com