Rapid separation device and separation method for plant root system tissues and attached soil samples

Abstract

The invention discloses a rapid separation device and separation method for plant root system tissues and attached soil samples. The device comprises a main connecting rod, a first weighting block isfixedly connected to the bottom end of the main connecting rod, the bottom end of the first weighting block is rotationally connected with a base through a ball bearing, a rotating assembly is installed at the top end of the main connecting rod, and a root system tissue fixing assembly is installed on the side wall of the main connecting rod. According to the invention, the rapid separation operation can be provided, operation is directly carried out in situ, a sample does not need to be transferred into a laboratory for separation operation, the operation can be carried out by a single person, no additional power instrument is needed, and the operation method is simple; meanwhile, the device can be suitable for a taproot system and also can be used for a fibrous root system, and operationand performance change caused by the shape and size of the plant root system are avoided; the separated root system sample can be directly used for subsequent experiments after simple pretreatment, and the attached soil sample is a sample in an in-situ environment, so that the requirements of a culture experiment are met.

Description

Technical field [0001] The invention relates to the technical field of separation, in particular to a rapid separation device and a separation method for plant root tissues and attached soil samples. Background technique [0002] Plant roots support the growth and erection of plants, and are also the core of the growth and reproduction of other symbiotic microorganisms in the rhizosphere environment. A large number of studies on the analysis of plant root tissue or root attachment soil samples, the first step is to separate the root tissue and attachment soil samples, and then the plant roots and attachment soil samples can be investigated with different test methods. In actual operation, researchers often separate the entire soil sample (or sediment sample) covering the root tissue from the original position, and then separate the root tissue from the attached soil sample by cleaning, ultrasound, and high-speed centrifugation in the laboratory . During this period, it takes a ...

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Problems solved by technology

[0004] Purpose of the invention: The purpose of the present invention is to address the above-mentioned deficiencies in the prior art, to provide a rapid separation device and separation method for plant root tissue and attached soil samples, so as to solve the problem that the existing separation methods cannot be performed in situ and require complicated operations. and the technical problem of not being able to accurately locate microbial samples of the rhizosphere environment for subsequent culture

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