Adjuvant for chicken Newcastle disease live vaccine, and preparation method of adjuvant
A technology for chicken Newcastle disease and live vaccine, which is applied in the field of adjuvant and preparation of chicken Newcastle disease live vaccine, can solve the problems of lack of pertinence, high virulence, poor effect and the like, and achieves the effects of good emulsifying effect, low toxicity and promoting metabolic function.
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Embodiment 1
[0033] Paraffin 4g, lanolin 8g, BCG 3.5g, Mycobacterium tuberculosis 6.5g, levamisole 6g, vitamin B 4g, emulsifier 2g, phosphate buffer 12g.
[0034] Put lanolin and paraffin oil in a container, mix them evenly with ultrasonic waves, sterilize under high pressure, and store them below 4°C for later use; then put BCG and Mycobacterium tuberculosis into different culture media, add normal saline and shake well, Control the temperature at room temperature for 5-20 minutes, then mix it with step S1, add levamisole and vitamin B respectively for mixing, and send it to an incubator, control the temperature at 35-55°C for 2-4 hours; then mix Processing, then add emulsifier to mix and stir, and add phosphate buffer, keep 60-72°C during the stirring process, last for 30 minutes, and finally disinfect the solution, after the treatment is completed, let it stand for 15 minutes to complete the processing, according to 0.5ml Servings are packed.
[0035] Safety inspection: use 20 broiler ...
Embodiment 2
[0037] Paraffin 2g, lanolin 3g, BCG 1.8g, Mycobacterium tuberculosis 2.7g, levamisole 2.5g, vitamin B1.5g, emulsifier 0.5g, phosphate buffer 8g.
[0038] Put lanolin and paraffin oil in a container, mix them evenly with ultrasonic waves, sterilize under high pressure, and store them below 4°C for later use; then put BCG and Mycobacterium tuberculosis into different culture media, add normal saline and shake well, Control the temperature at room temperature for 5-20 minutes, then mix it with step S1, add levamisole and vitamin B respectively for mixing, and send it to an incubator, control the temperature at 35-55°C for 2-4 hours; then mix Processing, then add emulsifier to mix and stir, and add phosphate buffer, keep 60-72°C during the stirring process, last for 30 minutes, and finally disinfect the solution, after the treatment is completed, let it stand for 15 minutes to complete the processing, according to 0.8ml Servings are packed.
[0039] Safety inspection: use 20 broi...
Embodiment 3
[0041] Paraffin 3g, lanolin 5g, BCG 2g, Mycobacterium tuberculosis 4g, levamisole 3g, vitamin B 2g, emulsifier 1.5g, phosphate buffer 10g.
[0042]Put lanolin and paraffin oil in a container, mix them evenly with ultrasonic waves, sterilize under high pressure, and store them below 4°C for later use; then put BCG and Mycobacterium tuberculosis into different culture media, add normal saline and shake well, Control the temperature at room temperature for 5-20 minutes, then mix it with step S1, add levamisole and vitamin B respectively for mixing, and send it to an incubator, control the temperature at 35-55°C for 2-4 hours; then mix Processing, then add emulsifier to mix and stir, and add phosphate buffer, keep 60-72°C during the stirring process, last for 30 minutes, and finally disinfect the solution, after the treatment is completed, let it stand for 15 minutes to complete the processing, according to 0.8ml Servings are packed.
[0043] Safety inspection: use 20 broiler chi...
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