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Eluent

An eluent, ethanol technology, applied in the field of chromatographic separation, can solve problems such as low elution efficiency of the eluent

Inactive Publication Date: 2020-08-28
CHANGDE ZHENGYANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides an eluent, aiming to solve the problem of low elution efficiency of existing eluents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] An eluent provided in the embodiment of the present invention is composed of the following components in volume percentage: 15% water, 30% ethanol, 10% ethyl acetate, 6% butyl acetate, 3% n-propanol, methyl 9% isobutyl ketone, 6% tetrahydrofuran, 5% isopropanol, 7% chloroform, 9% methyl ethyl ketone.

[0021] The eluent provided in the embodiment of the present invention is used to elute the eluent containing solanesol, that is, the eluent provided in the embodiment of the present invention is pumped into the above-mentioned chromatographic column, and the filler in the chromatographic column absorbs the above-mentioned paste body. It has been determined that the elution rate of the target substance with the eluent provided in the embodiment of the present invention is 80.23%.

Embodiment 2

[0023] An eluent provided in the embodiment of the present invention is composed of the following components in volume percentage: 10% water, 48% ethanol, 6% ethyl acetate, 5% butyl acetate, 2% n-propanol, methyl 5% isobutyl ketone, 3% tetrahydrofuran, 5% isopropanol, 7% chloroform, 9% methyl ethyl ketone.

[0024] The eluent provided in the example of the present invention is used to elute the eluent containing polyphenols, and the order of use of the eluent provided in the example of the present invention is after the eluent provided in Example 1, that is, the use of the eluent provided in the invention After the eluent provided in Example 1 elutes the eluent containing solanesol, use the eluent provided in the embodiment of the present invention to pump into the chromatographic column and elute the eluent containing the above-mentioned polyphenols . It is determined that the elution rate of the target substance provided by the eluent provided in the embodiment of the prese...

Embodiment 3

[0026] An eluent provided in the embodiment of the present invention is composed of the following components in volume percentage: 1% water, 43% ethanol, 10% ethyl acetate, 6% butyl acetate, 3% n-propanol, methyl 9% isobutyl ketone, 6% tetrahydrofuran, 5% isopropanol, 7% chloroform, 10% methyl ethyl ketone.

[0027] The eluent provided in the embodiment of the present invention is used to elute the eluent containing macromolecular substances, wherein the macromolecular substances include alkanes, alcohols and waxy substances with a carbon number ≥ 45. The order of use of the eluent provided in the example is after the eluent provided in the second example, that is, after the eluent containing the polyphenols is eluted using the eluent provided in the second example of the present invention, and then the present invention is used. The eluent provided in the examples is pumped into the chromatographic column and the eluent containing the above-mentioned macromolecular substances...

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PUM

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Abstract

The invention is suitable for the technical field of chromatographic separation, and provides an eluent which comprises the following components: water, ethanol, ethyl acetate, butyl acetate, n-propanol, methyl isobutyl ketone, tetrahydrofuran, isopropanol, trichloromethane and methyl ethyl ketone. According to the eluent, the solanesol, the polyphenol substances and the macromolecular substancescan be respectively eluted to obtain eluent by selecting proper solvents to be mixed according to different proportions so that the solanesol with higher purity and effective substances contained in other tobaccos can be separated in subsequent steps.

Description

technical field [0001] The invention belongs to the technical field of chromatographic separation, and in particular relates to an eluent. Background technique [0002] In elution chromatography, the process in which the mobile phase carries the components to be measured to move forward in the chromatographic column and out of the chromatographic column is called elution, and the mobile phase used is called the eluent. [0003] Solanesol is an active ingredient of polyterpenoids contained in tobacco. It is an unsaturated polyprenyl alcohol. It has the function of lipid antioxidant and eliminating free radicals in antibodies. It is a pharmaceutical intermediate of traditional Chinese medicine and can be used for synthesis Vitamin K2, coenzyme Q10, anti-cancer and anti-AIDS drugs. Coenzyme Q10, which has been produced and widely used in many developed countries, must rely on high-purity solanesol as a raw material. [0004] The process for extracting solanesol from tobacco m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/42
CPCB01D15/426
Inventor 曾勇黄平易松山杨鸿波陈德亮陈孟军
Owner CHANGDE ZHENGYANG BIOTECH CO LTD