New trichoderma with saline-alkaline tolerance and application of new trichoderma

A salt-alkali and Trichoderma-resistant technology, applied in the biological field, can solve the problem of less Trichoderma and its preparations

Active Publication Date: 2020-09-04
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the biocontrol and growth promotion mechanism of Trichoderma is still in the stage of continuous improvem

Method used

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  • New trichoderma with saline-alkaline tolerance and application of new trichoderma
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  • New trichoderma with saline-alkaline tolerance and application of new trichoderma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation and identification of tidal flat Trichoderma TUCIM 10301

[0030] Tidal flat Trichoderma TUCIM 10301 was isolated from the rhizosphere soil of Phragmites reed in coastal flats of Dafeng Port, Jiangsu Province, and the preservation number is CGMCC NO.19611.

[0031] 1.1 Morphological characteristics of tidal flat Trichoderma TUCIM 10301

[0032] 1.1.1 Test medium

[0033] PDA: potato dextrose agar medium;

[0034] CMA: corn flour agar medium;

[0035] SNA: Synthetic Low Nutrient Agar Medium (Recipe: KH 2 PO 4 1.0g l -1 ; KNO 3 1.0g l -1 ;MgSO 4 ·7H 2 O0.5g l -1 ;KCl 0.5g l -1 ; Glucose 0.2g l -1 ;Sucrose 0.2g l -1 ;Agar powder 20g l -1 ; pH adjusted to 7.0)

[0036] 1.1.2 Colony morphology

[0037] Inoculate the TUCIM 10301 strain of tidal flat Trichoderma TUCIM 10301 on the above-mentioned medium, and then culture it under dark conditions at 25°C for 3 days to observe its morphology. figure 1 ):

[0038] Colony: Trichoderma TUCIM...

Embodiment 2

[0044] Embodiment 2: the saline-alkali tolerance of tidal flat Trichoderma TUCIM 10301

[0045] Phragmites reed can grow normally in coastal saline soil with a salt content of 1.5% and pH = 8.5, so Trichoderma isolated from the rhizosphere soil samples of Phragmites is highly likely to tolerate high-salt and high-alkaline environments. From the Trichoderma screened, select the strains with fast growth and many spores to carry out NaCl and pH tolerance test as follows:

[0046] 2.1 Salt tolerance test:

[0047] Taking the tidal flat Trichoderma TUCIM 10301 strain as the main research object, another two fast-growing, spore-forming Trichoderma strains were selected to conduct salt tolerance tests together. Inoculate the three bacteria on the PDA plate at the same time, culture at 28°C for 7 days, after producing a large number of conidia, scrape the spores under sterile conditions and dilute them to OD with sterile water 600 = 0.1. First distribute 30% Murashige Skoog medium ...

Embodiment 3

[0052] Example 3: Inhibitory effect of tidal flat Trichoderma TUCIM 10301 on various plant pathogenic fungi

[0053] Four plant pathogenic fungi were selected, including Pestalotiopsis fici TUCIM 5788, Rhizoctonia solani TUCIM 3753, Fusarium oxysporum TUCIM4848, Alternaria alternata TUCIM 10233), the above-mentioned phytopathogenic bacteria were inoculated on one side of a 9 cm PDA plate with a sterilized hole puncher with a diameter of about 5 mm, and inoculated Trichoderma TUCIM 10301 on the other side of the plate after 24 hours, and cultivated in the dark at 25 ° C. On 14d, take pictures to record the situation of fungal confrontation.

[0054] The results show( Figure 4 ), Trichoderma TUCIM 10301 can inhibit the growth of Alternaria and Rhizoctonia solani colonies, limit the colony radius to 1.5-2.0cm, and completely cover the colonies of these two pathogenic fungi, producing A small amount of Trichoderma spores; Trichoderma TUCIM 10301 antagonizes Fusarium oxysporum a...

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Abstract

The invention discloses new trichoderma with saline-alkaline tolerance and application of the new trichoderma. According to the new trichoderma TUCIM 10301, the preservation number is CGMCC 19611. Thestrain is a new species of trichoderma sp. and is finally named Trichoderma arenerea sp.nov. The Trichoderma arenerea sp.nov. TUCIM 10301 can tolerate saline stress caused by 1.5M (8%) salt concentration and alkaline stress with pH value reaching 9.0. Under the stress of different salt concentrations, the Trichoderma arenerea sp.nov. TUCIM 10301 removes influence of salt on the plant growth inhibition effect, and compared with control without inoculation, the inoculated trichoderma TUCIM 10301 has the advantages of increasing biomass of crops by 37% and 81% under the condition of NaCl contentof 0.5% and 0.75% respectively and promoting root elongation and lateral root differentiation of crops.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a saline-alkali-resistant Trichoderma neophylla and its application. Background technique [0002] my country is rich in saline soil resources, which can be divided into four major types: coastal saline areas, Huanghuai-Hai plain saline areas, desert and desert grassland saline areas, and grassland saline areas. Among them, the total area of ​​tidal flat saline soil in Jiangsu is 687,300 hectares, accounting for about 1 / 4 of the total area of ​​tidal flats in China, and the net increase area is about 1,100 hectares per year. Although the reclamation and development of coastal tidal flats in northern Jiangsu has a long history, at present, salinization is still one of the main obstacles restricting the development and utilization of tidal flat soils in northern Jiangsu. It has heavy salinity, poor water and fertilizer retention, unstable structure, and low microbial activity. , it is d...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCC12R2001/885C12N1/145
Inventor 蔡枫丁明月伊瑞娜·杜鲁兹尼娜
Owner NANJING AGRICULTURAL UNIVERSITY
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