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Identification method and application of Japanese claw fern herb

A technology of golden flower and fingerprints, which is applied in the field of identification of golden flower, and can solve the problems of unseen quality evaluation

Inactive Publication Date: 2020-09-08
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Auranthus microphylla mainly focuses on the identification of crude drugs, gametophyte development, separation of chemical components and pharmacological effects, etc., and there is no research on quality evaluation.

Method used

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  • Identification method and application of Japanese claw fern herb
  • Identification method and application of Japanese claw fern herb
  • Identification method and application of Japanese claw fern herb

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Preparation of test solution

[0041]Thirteen batches of medicinal material samples were identified as the whole herb of Onychium japonicum (Thunb.) Kze., a plant in the family Pteridaceae. The specific source information is shown in Table 1.

[0042] Table 1 Genetic source information of Goldenflower microphylla

[0043]

[0044] Grind the whole herb samples of S1-S13 Golden Flower Herb into powder and pass through a No. 3 sieve; respectively take 1.0 g of Golden Flower Flower powder, weigh them accurately, put them in a round-bottomed flask, and add methanol-acetic acid-water (45 :5:50) solution 30mL, weighed, heated and refluxed in a water bath for 1.5h, let cool to room temperature, weighed again and supplemented the quality, shaken up, filtered, and the filtrate was taken as the test solution.

[0045] (2) Preparation of reference substance solution

[0046] Take appropriate amounts of protocatechuic acid, vanillic acid, caffeic acid, and cichoric acid ref...

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Abstract

The invention discloses an identification method and application of Japanese claw fern herb, and belongs to the technical field of pharmaceutical analysis. The identification method comprises a preparation method of a test solution and high performance liquid chromatography detection conditions. Processing is performed by using traditional Chinese medicine fingerprint spectrum similarity softwareto obtain a fingerprint spectrum of the Japanese claw fern herb; and importing related chromatographic data into SPSS software to carry out clustering analysis and principal component analysis, and arelated analysis result is obtained. According to the method, a common mode of the HPLC fingerprint spectrum of the Japanese claw fern herb is established, 11 common peaks are identified, wherein fourcommon peaks are known components, and most information of medicinal material index components is covered. The method is simple, convenient, rapid and good in reproducibility, fills up the blank of aquality control method of the Japanese claw fern herb and has high application value.

Description

technical field [0001] The invention relates to the technical field of drug analysis, which analyzes the main components in the medicinal materials and evaluates the quality of the medicinal materials, and more specifically relates to a method for identifying Golden Flower Herb and its application. Background technique [0002] Golden Flower Grass comes from Onychium japonicum (Thunb.) Kze., a plant in the family Fernaceae, also known as Golden Flower Fern, Small Pheasant Tail, Japanese Black Fern, etc. It is a commonly used traditional Chinese medicine in Yao and Zhuang areas of Guangxi. Its taste is bitter, and has the effects of clearing away heat and detoxification, promoting dampness and stopping bleeding. It is mainly used to treat wind-heat cold, lung-heat cough, acute gastroenteritis, jaundice, blood in the stool, etc. At present, the researches on Aurea microphylla mainly focus on the identification of crude drugs, gametophyte development, separation of chemical co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86
CPCG01N30/8686
Inventor 陈锋李嘉张颖杨海船何春欢张赟赟巫凯
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI