Multi-component subunit vaccine for African swine fever, and preparation method and application of multi-component subunit vaccine

A subunit vaccine and African swine fever technology, applied in the field of animal vaccines and veterinary biological products, can solve the problems of complex pathogenic mechanism, biological safety hidden dangers, immune interference, and poor immune effect, so as to achieve no immune interference and prevent And protect infection, meet the effect of industrial application

Pending Publication Date: 2020-09-15
NOVO BIOTECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problems to be solved by the present invention: one is to develop a large-scale industrial production, good quality, and capable of preventing The subunit vaccine of African swine fever virus; the second is to overcome the use of attenuated African swine fever vaccine may cause African swine fever virus and other viral genomes or residual nu

Method used

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  • Multi-component subunit vaccine for African swine fever, and preparation method and application of multi-component subunit vaccine
  • Multi-component subunit vaccine for African swine fever, and preparation method and application of multi-component subunit vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the preparation of African swine fever virus CD2V protein, P54 protein, P30 protein and P72 protein

[0027] 1.1 Preparation of African swine fever virus CD2V protein: refer to the preparation method of African swine fever CD2V protein in the invention patent of application number 201910004596.5 or 201910069838.9 or the preparation method in other patents or literatures to prepare African swine fever CD2V protein, and the full-length can be prepared according to actual needs African swine fever CD2V protein or CD2V-Fc fusion protein.

[0028] 1.2 Preparation of African swine fever virus p54 protein: refer to the published literature on the expression of African swine fever virus p54 protein and the preparation of monoclonal antibody (Feng Chunyan et al., 2016, China Animal Quarantine) or the prokaryotic expression and analysis of African swine fever virus p54 recombinant protein. Its monoclonal antibody preparation (Feng Yao, 2014, master's thesis of Yangzho...

Embodiment 2

[0031] Embodiment 2: the preparation of African swine fever subunit vaccine

[0032] The consumables and materials used for the preparation of vaccines need to undergo aseptic treatment in advance, and the preparation process is completed in a biological safety cabinet or other instruments or environments that can ensure that the entire preparation process is sterile.

[0033] 2.1 Vaccine preparation (taking the mixture of four proteins as an example)

[0034] Water phase preparation: According to the CD2V, p54, TF-P72, TF-P30 protein content in the vaccine, use PBS (or normal saline) to dilute the CD2V, p54, TF-P72, TF-P30 protein to an appropriate concentration, which is the water phase;

[0035] Preparation of oil phase: According to the total amount of prepared vaccine, according to the weight ratio of antigen phase and adjuvant is 1:1, and the volume ratio is 46:54, measure an appropriate amount of ISA 201 VG adjuvant;

[0036] Emulsification: Preheat both the water phas...

Embodiment 3

[0038] Embodiment 3: African swine fever subunit vaccine immunization experiment

[0039] 3.1 Vaccine preparation: Prepare proteins and vaccines according to the methods of Examples 1 and 2, and the specific vaccine information is shown in Table 1 below:

[0040] Table 1

[0041]

[0042] 3.2 Immunization experiment: Screen 40 piglets aged 28-35 days (CSFV, PCV2, PRRSV antigen antibody negative), randomly divide into 8 groups, 5 piglets in each group, one group serves as blank control group, and the other 7 groups are immunized with vaccine 1 and vaccine respectively 2. Vaccine 3, Vaccine 4, Vaccine 5, Vaccine 6, Vaccine 7. The blank control group was intramuscularly injected with 1ml of normal saline each time. In addition, 1ml of the corresponding vaccine was intramuscularly injected into the 7 groups of immunization groups each time, and a booster immunization was given three weeks after the initial immunization. Serum was collected before immunization, before the seco...

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Abstract

The invention discloses a multi-component subunit vaccine for African swine fever, and a preparation method and application of the multi-component subunit vaccine, and belongs to the technical field of animal vaccines and veterinary biological products. The multi-component subunit vaccine comprises an African swine fever virus surface envelope protein CD2V, at least one protein selected from an African swine fever virus P72 protein, an African swine fever virus P30 protein and an African swine fever virus P54 membrane structure protein, and a pharmaceutically acceptable adjuvant, wherein the African swine fever virus surface envelope protein CD2V is an African swine fever virus CD2V protein without a transmembrane region, or a fusion protein CD2V-Fc of an African swine fever virus CD2V extracellular region protein and a swine antibody Fc. The multi-component subunit vaccine provided by the invention has strong immunogenicity and good safety, and can significantly induce humoral immuneresponse.

Description

technical field [0001] The invention relates to a multicomponent subunit vaccine of African swine fever, a preparation method and application thereof, and belongs to the technical field of animal vaccines and veterinary biological products. Background technique [0002] African swine fever is an acute, febrile and fatal disease caused by African swine fever virus (ASFV). Its symptoms are similar to typical swine fever. Pigs are the only hosts. Animal diseases that must be reported under the OIE Animal Health Code. my country also lists it as a class of animal diseases in the list of animal diseases. The disease was introduced into my country in August 2018, causing great losses to my country's pig industry. [0003] African swine fever virus belongs to the African swine fever virus family and a member of the African virus genus. Its characteristics are similar to those of the iridoviridae family, and it has some characteristics of iridoviruses and poxviruses. ASFV is a ...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61P31/20
CPCA61K39/12A61P31/20A61K2039/552A61K2039/70C12N2710/12034
Inventor 钱泓吴有强张强吴素芳车影
Owner NOVO BIOTECH CORP
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