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Vaccine of classical swine fever-porcine circular double subunit and its preparation method and application

A technology of porcine circle and double subunit, which is applied in the field of swine fever-porcine circle double subunit vaccine and its preparation, can solve the problems of difficulty in rational arrangement of immunization procedures for various diseases, potential biological safety hazards, immune interference, etc., and achieve Prevention and protection of infection, strong immunogenicity, and cost-saving effects

Active Publication Date: 2022-05-27
NOVO BIOTECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problems to be solved by the present invention are as follows: one is to develop a vaccine capable of large-scale industrial production, good quality, no immune interference and capable of simultaneously preventing the infection of swine fever virus and porcine circovirus type 2; The problems of multi-injection, multi-dose, and multi-disease immunization procedures in the field are difficult to arrange reasonably; the third is to overcome the possibility of using the live swine fever vaccine to develop combined vaccines that may lead to the remaining nucleic acid in the genome of swine fever virus and other viruses or other vaccines. Recombination causes the mutation of the CSF virus, so there are great biological safety hazards and possible immune interference problems; the fourth is to avoid the need to separate the CSF virus and other disease antigens when using live CSF vaccines to prepare dual vaccines and the trouble of mixing when using

Method used

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  • Vaccine of classical swine fever-porcine circular double subunit and its preparation method and application
  • Vaccine of classical swine fever-porcine circular double subunit and its preparation method and application
  • Vaccine of classical swine fever-porcine circular double subunit and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of swine fever virus E2 protein and porcine circovirus type 2 Cap protein

[0030] 1.1 Preparation of swine fever virus E2 protein: refer to the preparation method of swine fever swine E2 protein preparation method in the patent or other patent documents with application number of 200810178235.4 or 200310103408.3 or 200810150304.0 or 201310300549.8 or 200810178235.4 or 201610625392.X or 201510187995.1 E2 protein, full-length swine fever E2 protein or truncated swine fever E2 protein can be prepared according to actual needs.

[0031] 1.2 Preparation of porcine circovirus type 2 Cap protein: refer to the preparation method of porcine circovirus type 2 Cap protein in the invention patent with the application number of 201310050003.1 or 201210270504.6 or 201110100331.9 or 201110053536.6 or 201010618223.6 or the preparation method in other patents or documents to prepare pigs For circovirus type 2 Cap protein, full-length porcine circovirus type 2 Cap...

Embodiment 2

[0032] Example 2: Preparation of swine fever-porcine cyclodiplex vaccine

[0033] The consumables and materials used to prepare the vaccine need to be pre-sterilized, and the preparation process is completed in a biological safety cabinet or other equipment or environment that can ensure that the entire preparation process is sterile.

[0034] 1. Preparation of Montanide GEL 01 PR (adjuvant): According to the mass ratio of antigen liquid and adjuvant to 10:1, weigh 20g (about 20ml) of adjuvant and put it in a pre-prepared reagent bottle, seal it and wait for use.

[0035] 2. According to the mass ratio of antigen liquid and adjuvant being 10:1, the total mass of antigen liquid is 200g (about 200ml). According to the concentration of swine fever virus E2 protein and porcine circovirus type 2 Cap protein concentration and the content of each protein in the vaccine, calculate the volume of E2 protein and Cap protein; use PBS buffer or other buffer to supplement the total mass of ...

Embodiment 3

[0040] Example 3: Swine fever-porcine cyclodiplex vaccine immunization experiment

[0041] 3.1 Vaccine preparation: The protein and vaccine were prepared according to the methods of Examples 1 and 2. The specific vaccine information is shown in Table 2 below:

[0042] Table 2

[0043]

[0044] 3.2 Immunization experiment: Screen 35 piglets aged 28-35 days (negative for CSFV and PCV2 antigen and antibody) and randomly divide them into 7 groups with 5 pigs in each group. The blank control group was injected with 1ml of normal saline each time, and the other 6 groups of immunization groups were injected with 1ml of the corresponding vaccine each time. Three weeks after the initial immunization, the immunization was boosted once. Serum was collected before immunization, before the second immunization, and 21 days after the second immunization. Antibody titers.

[0045] The experimental results are as Figure 1a The test results of IDEXX swine fever antibody detection kit show...

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Abstract

The invention discloses a vaccine of swine fever-porcine circular double subunit and its preparation method and application, belonging to the technical field of animal vaccines and veterinary biological products. The vaccine comprises classical swine fever virus E2 protein, porcine circovirus type 2 Cap protein and a pharmaceutically acceptable adjuvant. The method for preparing the vaccine comprises the following steps: 1) preparing classical swine fever virus E2 protein and porcine circovirus type 2 Cap protein; 2) preparing the classical swine fever virus E2 protein and porcine circovirus type 2 Cap protein prepared in 1) 3) Mix and stir the antigen solution and Montanide GEL 01PR adjuvant according to the mass ratio of 10:1. The vaccine of the present invention has the advantages of strong immunogenicity, good safety, no immune interference, no potential biological safety hazards of virus change, and the ability to fundamentally purify swine fever virus and porcine circovirus type 2; and through the Vaccine immunization can achieve the effect of "one shot, two defenses", saving time, labor and cost.

Description

technical field [0001] The invention relates to a swine fever-porcine cyclodiplex vaccine, a preparation method and application thereof, and belongs to the technical field of animal vaccines and veterinary biological products. Background technique [0002] Swine fever is an acute, febrile and fatal disease caused by Classical Swine Fever Virus (CSFV). Swine fever is characterized by highly contagious, acute onset, high fever, and extensive hemorrhage, infarction and necrosis caused by degeneration of small blood vessel walls. Domestic and wild pigs are its only natural hosts. The World Organization for Animal Health (OIE) classifies it as a Class A infectious disease, and my country's Animal Epidemic Prevention Law lists it as a Class I infectious disease. Swine fever is one of the major diseases that endanger the development of my country's pig industry. [0003] CSFV is a member of the Flaviviridae family and Pestivirus genus, and is a single-stranded linear RNA virus. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/187A61K39/12A61P31/20A61P31/14
CPCA61K39/12A61K39/39A61K2039/70A61K2039/552C12N2750/10034C12N2770/24334
Inventor 钱泓吴有强查银河
Owner NOVO BIOTECH CORP
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