Tachypleus tridentatus blood quality monitoring method based on continuous blood sampling

Abstract

The invention discloses a tachypleus tridentatus blood quality monitoring method based on continuous blood sampling. According to the method, a copper element content, a hemocyte count, a plasma protein content and a hemolymph protein content in tachypleus tridentatus blood are used as blood quality indexes for monitoring tachypleus tridentatus; the quality of the tachypleus tridentatus blood is monitored by detecting the content of the copper element in the tachypleus tridentatus blood and calculating other indexes; and the monitoring method comprises the steps of obtaining a sample, treatingthe sample, detecting the content of the Cu element in the sample, calculating a result and determining a health standard of the tachypleus tridentatus blood quality. The method is simple, convenient, efficient, rapid, time-saving, labor-saving and money-saving, a blood sampling amount of the tachypleus tridentatus blood quality is only 1-3 ml, once the method is implemented in production, an influence of improper blood sampling on tachypleus tridentatus health can be reduced, and the method has important significance in utilization and protection of the tachypleus tridentatus.

Description

technical field [0001] The invention relates to a blood quality monitoring method of crustacean marine animals, in particular to a Chinese horseshoe crab blood quality monitoring method based on continuous blood collection, which belongs to the field of development and utilization of marine medicinal resources. Background technique [0002] There are only 4 kinds of horseshoe crabs left in the world, namely the American horseshoe crab (Limulus polyphemus) on the east coast of the Americas, the Chinese horseshoe crab (Tachypleus tridentatus) in Asia (also known as three-spine horseshoe crab, Chinese horseshoe crab, and Oriental horseshoe crab), and the southern horseshoe crab (Tachypleus gigas). ) and round-tailed horseshoe crab (Carcinoscorpius rotundicauda). Among the four kinds of Limulus, only Chinese Limulus and American Limulus can produce Limulus reagent, so the Limulus reagent can be divided into two types according to the source, one is Chinese Limulus reagent (Tachy...

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems that the existing technology has high requirements for experimental equipment, and at the same time consumes a large amount of materials such as test kits, and requires high manpower, material resources, and financial resources to monitor blood quality in the process of producing Limulus reagent, the present invention provides a A small amount of Chinese horseshoe crab blood is taken as a sample during each blood collection process. Only the copper content in the Chinese horseshoe crab blood is detected, and the physiological and biochemical indicators such as the number of blood cells, plasma protein content, and hemolymph protein content are calculated based on the copper content to monitor Changes in the blood quality of Chinese horseshoe crabs, establishing a blood quality monitoring method for Chinese horseshoe crabs based on continuous blood sampling

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