Licorice extract and essential oil composite nanoemulsion preparation and preparation method
A technology of licorice extract and emulsion preparation, applied in directions such as drug combination, pharmaceutical formula, emulsion delivery, etc., to achieve the effects of mild preparation conditions, fresh breath, and guaranteeing biological safety
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Embodiment 1
[0019] Embodiment 1: see figure 1 , the specific preparation steps of a composite nanoemulsion of licorice crude extract and essential oil are as follows:
[0020] S1. Accurately weigh 0.5g of licorice crude extract and 0.5g of essential oil, place them in a centrifuge tube, vortex for 5-10min, make the two fully mixed, and obtain a milky yellow oily liquid, which is the oil phase;
[0021] S2. Accurately weigh 5g of surfactant Tween 20 and 50g of deionized water, place them in an 80ml beaker, place the beaker on a magnetic stirrer, set the temperature to 25°C, adjust the speed to 500rpm, and stir for 30min to obtain The clarified aqueous solution is the external water phase;
[0022] S3. Then slowly add the oil phase drop by drop, and initially disperse the oil phase into the outer water phase until all the components are completely dispersed, and then prepare the suspension with high shear. The shear conditions are as follows: the rotation speed is 12000rpm, the time After...
Embodiment 2
[0024] Example 2: Preparation of a composite nanoemulsion of licorice crude extract and essential oil
[0025] S1. Accurately weigh 0.5 g of licorice crude extract and 0.75 g of essential oil, place them in a centrifuge tube, vortex for 5-10 min, and mix the two fully to obtain a milky yellow oily liquid, which is the oil phase;
[0026] S2. Accurately weigh 5g of surfactant Tween 20 and 50g of deionized water, place them in an 80ml beaker, place the beaker on a magnetic stirrer, set the temperature to 25°C, adjust the speed to 500rpm, and stir for 30min to obtain The clarified aqueous solution is the external water phase;
[0027] S3. Then slowly add the oil phase drop by drop, and initially disperse the oil phase into the outer water phase until all the components are completely dispersed, and then prepare the suspension with high shear. The shear conditions are as follows: the rotation speed is 12000rpm, the time After 5 minutes, a yellow opaque emulsion was obtained, whic...
Embodiment 3
[0029] Example 3: Preparation of a composite nanoemulsion of licorice crude extract and essential oil
[0030] S1. Accurately weigh 0.5g of licorice crude extract and 1.0g of essential oil, place them in a centrifuge tube, vortex for 5-10min, make the two fully mixed, and obtain a milky yellow oily liquid, which is the oil phase;
[0031] S2. Accurately weigh 5g of surfactant Tween 20 and 50g of deionized water, place them in an 80ml beaker, place the beaker on a magnetic stirrer, set the temperature to 25°C, adjust the speed to 500rpm, and stir for 30min to obtain The clarified aqueous solution is the external water phase;
[0032] S3. Then slowly add the oil phase drop by drop, and initially disperse the oil phase into the outer water phase until all the components are completely dispersed, and then prepare the suspension with high shear. The shear conditions are as follows: the rotation speed is 12000rpm, the time After 5 minutes, a yellow opaque emulsion was obtained, whi...
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