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A high-voltage pulsed electric field-assisted enzymolysis method for producing taurine from mussel meat

A technology of high-voltage pulsed electric field and assisted enzymatic hydrolysis, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as instability, extraction method of taurine, low extraction rate, etc., to solve resource problems waste, the production process is green and efficient, and the effect of improving the extraction effect

Active Publication Date: 2022-02-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are methods for extracting taurine from meat products and seafood in the prior art, there is no special extraction method for extracting taurine from river mussels, and there is a technical gap. If the existing extraction technology is directly applied to In the process of extracting taurine from mussel meat, the extraction rate is low and unstable, so many parameters and steps in the extraction process need to be further innovated, optimized and improved

Method used

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  • A high-voltage pulsed electric field-assisted enzymolysis method for producing taurine from mussel meat
  • A high-voltage pulsed electric field-assisted enzymolysis method for producing taurine from mussel meat
  • A high-voltage pulsed electric field-assisted enzymolysis method for producing taurine from mussel meat

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Effect test

Embodiment 1

[0029] A high-voltage pulsed electric field-assisted enzymatic hydrolysis method for producing taurine, comprising the following steps:

[0030] (1) Raw material pretreatment:

[0031] Shell the clam meat to separate the viscera and meaty part, wash the meaty part, drain and homogenize it, and distribute it for equipment; (2) Wall breaking treatment:

[0032] Add distilled water to the pretreated mussel meat homogenate, the amount of distilled water added and the quality of the sample solution are added according to 1:1 (mL / g), and the mussel meat sample solution is obtained after intermittent treatment with a wall breaking machine for 2-3min. For distributing equipment;

[0033] (3) High-voltage pulsed electric field treatment:

[0034] Take 4g of mussel meat sample liquid, add 32mL of distilled water to the sample liquid for homogenization, adjust the flow rate parameter of the peristaltic pump to 7mL / min after homogenization, and then perform pulse electric field treatmen...

Embodiment 2

[0047] A high-voltage pulsed electric field-assisted enzymatic hydrolysis method for producing taurine, comprising the following steps:

[0048] (1) Raw material pretreatment:

[0049] Shell the clam meat to separate the viscera and meaty part, wash the meaty part, drain and homogenize it, and distribute it for equipment; (2) Wall breaking treatment:

[0050] Add distilled water to the pretreated mussel meat homogenate, the amount of distilled water added and the quality of the sample solution are added according to 1:1 (mL / g), and the mussel meat sample solution is obtained after intermittent treatment with a wall breaking machine for 2-3min. For distributing equipment;

[0051] (3) High-voltage pulsed electric field treatment:

[0052] Take 4g mussel meat sample liquid, add 32mL distilled water to the sample liquid for homogenization, adjust the flow rate parameter of the peristaltic pump to 7mL / min after homogenization, and then perform pulse electric field treatment, pul...

Embodiment 3

[0065] A high-voltage pulsed electric field-assisted enzymatic hydrolysis method for producing taurine, comprising the following steps:

[0066] (1) Raw material pretreatment:

[0067] Shell the clam meat to separate the viscera and meaty part, wash the meaty part, drain and homogenize it, and distribute it for equipment; (2) Wall breaking treatment:

[0068] Add distilled water to the pretreated mussel meat homogenate, the amount of distilled water added and the quality of the sample solution are added according to 1:1 (mL / g), and the mussel meat sample solution is obtained after intermittent treatment with a wall breaking machine for 2-3min. For distributing equipment;

[0069] (3) High-voltage pulsed electric field treatment:

[0070] Take 4g of mussel meat sample liquid, add 32mL of distilled water to the sample liquid for homogenization, adjust the flow rate parameter of the peristaltic pump to 7mL / min after homogenization, and then perform pulse electric field treatmen...

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Abstract

The invention relates to a method for producing taurine by enzymolysis of mussel meat assisted by a high-voltage pulse electric field. The purpose is to solve the current lack of methods for extracting taurine from mussel meat; Pulse electric field treatment, enzymatic hydrolysis, filtration, purification, crystallization and other steps effectively extract high-purity natural taurine products from mussel meat. Not only is the production process green and efficient, but also the test operation is simple, improving the extraction effect of taurine, The problems of resource waste and environmental pollution by mussel by-products are solved. The invention takes taurine extraction as an index, optimizes the four factors of electric field strength, pulse number, enzyme addition amount and enzymatic hydrolysis time, and greatly improves the quality of mussel meat. Extraction of taurine in .

Description

technical field [0001] The invention relates to a method for preparing taurine, in particular to a method for preparing taurine through enzymolysis of mussel meat assisted by a high-voltage pulse electric field. Background technique [0002] Taurine, whose scientific name is β-aminoethanesulfonic acid, is a sulfur-containing amino acid that exists in various tissues of animals in the form of free amino acids, and has physiological activity and various nutritional functions. At present, there are two production methods of taurine: chemical synthesis and natural extraction. Due to the existence of toxic substances in the raw materials and production process of chemical synthesis, people advocate the use of green and safe natural extraction to extract taurine. [0003] Although there are methods for extracting taurine from meat products and seafood in the prior art, there is no special extraction method for extracting taurine from river mussels, and there is a technical gap. If...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C303/44C07C309/14
CPCC07C303/44C07C309/14
Inventor 周亚军翟时敏詹妮陈艳张鸣镝郑磊李圣桡
Owner JILIN UNIV