Integrated molecular nucleic acid POCT device and method

Abstract

The invention discloses an integrated molecular nucleic acid POCT device and method. The integrated molecular nucleic acid POCT device is composed of a reagent card box and a card box support. Different cavities connected with one another are formed in the reagent card box, and each cavity corresponds to a valve switch for controlling opening and closing of liquid. The card box support provides switch opening and closing, piston motion, heating temperature measurement, card box existence determination and other functions for the reagent card box, and plays a role in supporting and positioning.The integrated device allows samples such as nasopharyngeal swabs, genital tract swabs, sputum and blood to be directly added without other treatment, and therefore integrated detection of sample input and result output is achieved. The device has the main characteristics that (1) the structure is simple, the cost is low, the operation is simple and convenient, and the whole process is finished in a closed manner, so that pathogenic bacteria and aerosol pollution are effectively prevented; (2) the integrated molecular nucleic acid POCT device is provided for field POCT; and (3) the cost performance is high, the preparation is simple, and grassroots and popular consumption are facilitated.

Description

technical field [0001] The invention relates to the technical field of biological devices, in particular to an integrated nucleic acid POCT detection device and method. Background technique [0002] In traditional nucleic acid detection, sample pretreatment, nucleic acid extraction, and nucleic acid detection need to be completed step by step independently, and each link needs to be operated in conjunction with specific equipment or instruments. The steps are complicated and need to be operated in a specific professional testing laboratory. Nucleic acid POCT in the field of molecular diagnosis is mostly designed using the principles of microfluidics and electrophoresis, resulting in complex preparation processes and high costs for its devices. In a special period like the new crown epidemic, real-time on-site testing cannot be achieved, resulting in a run on medical resources. Therefore, it is urgent to develop a molecular nucleic acid POCT device to realize the integrated ...

AI Technical Summary

Problems solved by technology

[0002] In traditional nucleic acid detection, sample pretreatment, nucleic acid extraction, and nucleic acid detection need to be completed step by step independently, and each link needs to be operated in conjunction with specific equipment or instruments. The steps are complicated and need to be operated in a specific professional testing laboratory

Nucleic acid POCT in the field of molecular diagnosis is mostly designed using the principles of microfluidics and electrophoresis, resulting in complex preparation processes and high costs for the devices.

In a special period similar to the new crown epidemic, real-time on-site testing cannot be achieved, resulting in a run on medical resources

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