Sodium lauroyl sarcosinate-bacitracin compound and composite oil displacement agent

A technology of sodium lauroyl sarcosinate and composite oil displacement agent, which is applied in the direction of drilling compositions, peptides, hybrid peptides, etc., which can solve the problem of reducing the effect of microbial oil displacement, the inability to achieve microbial oil displacement, and the weakening of microbial motility and other problems, to achieve the effect of improving synergistic reaction efficiency, operability and applicability, and simple method

Active Publication Date: 2020-10-13
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the addition of chemical oil displacement agents and their reaction process often destroy the physiological functions of microorganisms, reduce the effect of microbial oil displacement, and even cause large-scale death of microorganisms, resulting in

Method used

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  • Sodium lauroyl sarcosinate-bacitracin compound and composite oil displacement agent
  • Sodium lauroyl sarcosinate-bacitracin compound and composite oil displacement agent
  • Sodium lauroyl sarcosinate-bacitracin compound and composite oil displacement agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:

[0071] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;

[0072] (2) Bacteria were eluted with water at 40°C for 12 hours, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain the bacitracin compound (during the chromatographic separation, the retention time of the upper capsule solution was determined accor...

Embodiment 2

[0079] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:

[0080] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;

[0081] (2) Bacteria were eluted with water at 40°C for 12 hours, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain the bacitracin compound (during the chromatographic separation, the retention time of the upper capsule solution was determined accor...

Embodiment 3

[0085] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:

[0086] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;

[0087](2) Bacteria were eluted with water at 40°C for 12 hours, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain the bacitracin compound (during the chromatographic separation, the retention time of the upper capsule solution was determined accord...

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Abstract

The invention provides a sodium lauroyl sarcosinate-bacitracin compound and a composite oil displacement agent. A chemical structural formula of the sodium lauroyl sarcosinate-bacitracin compound is as shown in the specification. The invention provides the sodium lauroyl sarcosinate-bacitracin compound composite oil displacement agent containing the sodium lauroyl sarcosinate-bacitracin compound obtained by reacting sodium lauroyl sarcosinate with bacitracin. The composite oil displacement agent can be well compounded with oil recovery functional bacteria to form a microorganism-chemical composite oil displacement agent. The microorganism-chemical composite oil displacement agent has the degradation and viscosity reduction effects of microbial flooding on crude oil and has the emulsification and viscosity reduction effect of chemical flooding, the swept volume and the dispersion efficiency of the chemical oil displacement agent in an oil layer are increased in a biological carrying mode, the efficiency of a synergistic reaction of microorganisms and a chemical agent is effectively improved, and the purpose of 1+1>2 is achieved.

Description

technical field [0001] The invention belongs to the technical field of tertiary oil recovery, and relates to the preparation and application of a microbial-chemical composite oil displacement agent, in particular to a sodium lauroyl sarcosinate-bacitracin compound and a composite oil displacement agent. Background technique [0002] With the rapid development of the national economy, the demand for oil continues to increase, and the contradiction between oil consumption and supply has become more prominent. In order to solve the above contradictions, in addition to increasing exploration efforts and continuing to search for oil resources, increase crude oil recovery in developed oil fields Rate is also one of the effective ways. [0003] Microbial enhanced oil recovery (MEOR) is a technology that enhances oil recovery by utilizing the natural biochemical effects (biodegradation and metabolites) of living cells of microorganisms. By injecting microorganisms and / or nutrient s...

Claims

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Application Information

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IPC IPC(8): C07K5/083C09K8/58C09K8/584
CPCC07K5/0808C07K19/00C09K8/584
Inventor 宋文枫赵越章骏江航张帆
Owner PETROCHINA CO LTD
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