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Method for producing pleurotus ostreatus liquid strains from compound radix sophorae flavescentis alcohol precipitate

A technology of liquid strains and matrine alcohol, applied in botany equipment and methods, applications, plant cultivation, etc., to achieve the effects of promoting green sustainable development, increasing biomass, and facilitating nutrient utilization

Pending Publication Date: 2020-10-16
长治学院
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, so far, no high-density cultivation of Pleurotus ostreatus mycelia by fed-batch method has been reported.

Method used

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  • Method for producing pleurotus ostreatus liquid strains from compound radix sophorae flavescentis alcohol precipitate
  • Method for producing pleurotus ostreatus liquid strains from compound radix sophorae flavescentis alcohol precipitate
  • Method for producing pleurotus ostreatus liquid strains from compound radix sophorae flavescentis alcohol precipitate

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Embodiment Construction

[0032] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0033] A kind of method that utilizes compound flavescens alcohol precipitate to produce flat mushroom liquid spawn, comprises the following steps:

[0034] S1: Sterilize the fermentation medium in a solid tank. The bioreactor in this example uses a 10L mechanically agitated fermenter, put in a fermentation medium with an initial volume of 5L, cool to 28°C after sterilization, and ferment The medium includes the following components: 200mL / L compound flavescens alcohol precipitate hydrolyzate, 4g / L rice bran, 4g / L yeast extract, and 4g / L malt extract.

[0035] The preparation of the above compound flavescens alcohol p...

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Abstract

The invention relates to a method for producing pleurotus ostreatus liquid strains from compound radix sophorae flavescentis alcohol precipitate. The method comprises the following steps: putting hydrolysate of the compound radix sophorae flavescentis alcohol precipitate into a bioreactor for filled can sterilization, and inoculating a shake flask seed culture into the bioreactor, controlling theculture temperature in the bioreactor at 28 DEG C, and keeping pH at 5.5, supplying filtered sterile air into the bioreactor, keeping the dissolved oxygen concentration in a culture solution at 30% ofsaturated oxygen concentration, and performing fermenting for 72 hours; injecting a feed medium into the bioreactor at a constant speed, enabling the feed amount to be 1 / 5 of a fermentation medium, performing feed for 40 h, increasing the air flow rate with the increase of the size of the reactor to maintain 0.4 vvm, and continuing culture for 8 h after feed and finishing fermentation. Accordingto the method, the compound radix sophorae flavescentis alcohol precipitate is used as a material, and is used as a fermentation carbon source for liquid culture of radix sophorae flavescentis myceliaafter acid-heat pretreatment; and in the fermentation process, the mycelia biomass is increased by fed-batch culture.

Description

technical field [0001] The invention relates to the technical field of producing edible fungus strains, in particular to a method for producing liquid strains of oyster mushrooms by using compound flavescens alcohol precipitate. Background technique [0002] Compound Sophora flavescens injection, also known as Yanshu injection, is extracted and refined from flavescens flavescens and Baituling through percolation, alcohol precipitation and activated carbon decolorization. It is mainly used for anti-cancer, relieving cancer pain and bleeding, improving human immune function, etc. Compound Kushen Alcohol Precipitate (KP) is a muddy sediment produced during the alcohol precipitation process in the production of Compound Kushen Injection. At present, it is basically discarded as waste, resulting in waste of resources and environmental pollution. Water extraction of traditional Chinese medicine The alcohol precipitate contains a large amount of nutrients such as polysaccharides a...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/40A01G18/20
Inventor 靳志强
Owner 长治学院
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