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Cristata method of crassulaceae succulent plants

A technology of succulents and sedum, which is applied in the fields of botanical equipment and methods, plant regeneration, and horticultural methods. The effect of conjugation and increasing the number of cells

Active Publication Date: 2020-10-23
彩星(淄博)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has very large limitations: (1) Except for a few varieties, most plants are difficult to undergo natural embrittlement, and even for a few "easy" embellishment varieties, the embedding rate is only about 1% , the multiplication of embellished plants usually needs to wait for its natural embellishment and the multiplication speed is slow, and it is difficult for each natural embellished plant to produce more than 3 new plants per year, which is difficult to produce on a large scale
(2) This method can only increase the number of embellished plants, but it cannot be operated without embellished plants, which leads to the fact that most plants have no mutated products

Method used

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  • Cristata method of crassulaceae succulent plants
  • Cristata method of crassulaceae succulent plants
  • Cristata method of crassulaceae succulent plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 The embellishment method of Crassulaceae succulents

[0041] A method for embellishing sedum succulents, comprising the following steps:

[0042] (1) Disinfection of explants

[0043] Take the rainbow jade leaves without scars from the healthy growing rainbow jade. The petioles are as complete as possible. First rinse with water, then soak in 70% ethanol for 30 seconds, wash with 2% hypochlorous acid solution for 5 minutes, and finally use sterilized Wash with water 5 times, and dry the moisture on the surface of the leaves with absorbent paper;

[0044] (2) Culture of explants

[0045] Put the explants into 1 L of conjugation induction medium (MS medium, plus 5 mg 6-benzyl adenine, 0.2 mg naphthalene acetic acid, 30 g sucrose, 6.0 g agar, pH 5.8), and use LED lamps (Foshan lighting T5 integrated lamp) was given 12h of light every day for cultivation;

[0046] Among them, the lighting source ratio of LED lamps is 4000K (warm white light): 6500K (white l...

Embodiment 2

[0051] Embodiment 2 The embellishment method of Crassulaceae succulents

[0052] A method for embellishing sedum succulents, comprising the following steps:

[0053] (1) Disinfection of explants

[0054] Take blue apple leaves without scars from healthy blue apples. The petiole should be as complete as possible. Rinse with water first, then soak in 70% ethanol for 25 seconds, wash with 2% hypochlorous acid solution for 4 minutes, and finally wash with sterilized water 4 times, dry the moisture on the surface of the leaves with absorbent paper;

[0055] (2) Culture of explants

[0056] Put the explants into 1 L of conjugation induction medium (MS medium, plus 7 mg 6-benzyl adenine, 0.3 mg naphthalene acetic acid, 30 g sucrose, 6.0 g agar, pH 5.8), and use LED lamps (Foshan lighting T5 integrated lamp) was given 13h of light every day for cultivation;

[0057] Among them, the lighting source ratio of LED lamps is 4000K (warm white light): 6500K (white light) = 1:1.5.

[0058]...

Embodiment 3

[0062] Embodiment 3 The embellishment method of Crassulaceae succulents

[0063] A method for embellishing sedum succulents, comprising the following steps:

[0064] (1) Disinfection of explants

[0065] Take the leaves of Changshouhua without scars from the healthy growing Changshouhua, wash them with clean water first, then soak them in 70% ethanol for 35 seconds, wash them with 2% hypochlorous acid solution for 10 minutes, and finally wash them with sterilized water for 6 times. Absorb the water on the surface of the leaves with absorbent paper, cut into 1 cm wide strips for later use;

[0066] (2) Culture of explants

[0067] Put the explants into 1 L of conjugation induction medium (MS medium, plus 5 mg 6-benzyl adenine, 0.15 mg naphthalene acetic acid, 30 g sucrose, 6.0 g agar, pH 5.8), and use LED lamps (Foshan lighting T5 integrated lamp) was given 11 hours of light every day for cultivation;

[0068] Among them, the lighting source ratio of LED lamps is 3500K (war...

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Abstract

The invention discloses a cristata method of crassulaceae succulent plants. The method comprises the following steps of carrying out disinfection on explants of the crassulaceae succulent plants, culturing the explants in a cristata induction culture medium, carrying out rooting and seedling strengthening, and carrying out seedling hardening. According to the method, the development of the stem tip growth points of the plants is regulated and controlled by utilizing cytokinin, the differentiation repression of the meristems of the stem tip growth points is realized by manually controlling theconcentration of the cytokinin and auxin at the stem tip growth points of the plants, and the number of cells at the stem tip growth points is increased, so that the growth points are deformed into acristata line in a specialized growth manner; the method is simple and convenient to operate and low in cost, and the adventitious buds can be induced to generate the cristata plants by utilizing theleaves of the non-cristata crassulaceae succulent plants; and therefore, the method has good application value in crassulaceae succulent plant cristata.

Description

technical field [0001] The invention belongs to the technical field of ornamental plant seedling cultivation. More specifically, it relates to a method for embellishing succulents of the Crassulaceae family. Background technique [0002] Horticultural ornamental plants will undergo mutations during the growth process, some of which are caused by changes in genes, which can be inherited, and the mutated plants are new horticultural varieties; the other part of the mutation is caused by the environment, making plants produce Special, non-inheritable phenotypes that are different from their own genotypes, such variant phenotypes usually include cristata, brocade, petrochemical, etc. All of its offspring are variegated, which generally refers to the vast majority of plants that do not have variegated genes). Embellishment is caused by the variation of shoot apical growth point (SAM) of plants, and the embrittlement plant no longer has normal point-like shoot apical growth poin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 石可超李静徐书轩庄梦龙
Owner 彩星(淄博)生物科技有限公司