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Test strip based on release of labeled antibody on chromatographic membrane and preparation method

A technology for labeling antibodies and test strips, applied in the field of biomedical in vitro diagnostics, can solve the problems of high production cost of test strips, reduced test precision, difficult desorption of labeled antibodies, etc.

Pending Publication Date: 2020-10-30
北京华益精点生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the present invention provides a test strip based on the release of labeled antibodies on the chromatographic membrane and a preparation method to solve the instability of the release of labeled antibodies from the antibody binding pad, and the labeled antibodies are not easy to desorb on the chromatographic membrane. , the precision of the test is reduced, and the cost of test strips is high

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  • Test strip based on release of labeled antibody on chromatographic membrane and preparation method
  • Test strip based on release of labeled antibody on chromatographic membrane and preparation method
  • Test strip based on release of labeled antibody on chromatographic membrane and preparation method

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preparation example Construction

[0060] The preparation method of the test strip according to the embodiment of the present invention comprises:

[0061] Form a labeled antibody release line on the chromatographic membrane;

[0062] Form a capture antibody line on the chromatographic membrane;

[0063] forming a secondary antibody line on the chromatographic membrane, the capture antibody line is located between the labeled antibody release line and the secondary antibody line to obtain a test strip;

[0064] Wherein, the step of forming the labeled antibody release line on the chromatographic membrane includes:

[0065] mixing the labeled antibody with the adsorption solution to form a first mixed solution;

[0066] Using the first mixed solution to draw a line on the chromatographic membrane and dry it to form the labeled antibody release line;

[0067] The adsorption solution includes non-specific protein, surfactant and phosphate buffer;

[0068] Alternatively, the step of forming a labeled antibody r...

Embodiment 1

[0130] The nitrocellulose membrane is used for the chromatographic membrane, and the sample pad, the chromatographic membrane and the water-absorbing pad are assembled on the viscous base plate with their ends staggered;

[0131] Form the labeled antibody release line on the chromatographic membrane: mix the labeled antibody with the adsorption solution to form the first mixed solution; use the first mixed solution to draw a line on the chromatographic membrane and dry to form the labeled antibody release line; mix the first mixed solution with 1 μl / cm, speed 50mm / s, scratch on the nitrocellulose membrane close to the position where the sample pad is attached; bovine serum albumin 2% and Tween-204%, the pH value of phosphate buffer is 7, the first mixing The content of fluorescent molecule-labeled antibody in the solution can be 0.01g / L, and the labeled antibody is formed by using fluorescein-labeled urine microalbumin antibody;

[0132] Form the capture antibody line on the chr...

Embodiment 2

[0137] The nitrocellulose membrane is used for the chromatographic membrane, and the sample pad, the chromatographic membrane and the water-absorbing pad are assembled on the viscous base plate with their ends staggered;

[0138] Form the labeled antibody release line on the chromatographic membrane: mix the labeled antibody with the adsorption solution to form the first mixed solution; use the first mixed solution to draw a line on the chromatographic membrane and dry to form the labeled antibody release line; mix the first mixed solution with Draw 1 μl / cm, speed 50mm / s on the nitrocellulose membrane close to the position where the sample pad is attached; the adsorption liquid includes: bovine serum albumin 8% and Tween-20 8%, the pH value of phosphate buffer It can be 6.6, the content of the fluorescent molecule-labeled antibody in the first mixed solution can be 0.1g / L, and the labeled antibody is formed by using fluorescein-labeled urine microalbumin antibody;

[0139] For...

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Abstract

The invention provides a test strip based on release of a labeled antibody on a chromatographic membrane and a preparation method. The preparation method comprises the following steps that a labeled antibody release line is formed on the chromatographic membrane; a capture antibody line is formed on the chromatographic membrane; and a secondary antibody line is formed on the chromatographic membrane, and the capture antibody line is located between the labeled antibody release line and the secondary antibody line to obtain a test strip. The step of forming the labeled antibody release line onthe chromatographic membrane comprises the following substeps of mixing a labeled antibody with an adsorption solution to form a first mixed solution, scribing and drying the chromatographic membraneby using the first mixed solution to form a labeled antibody release line, wherein the adsorption liquid comprises non-specific protein, a surfactant and a phosphate buffer solution, or forming a non-specific protein layer on the chromatographic membrane, and forming a labeled antibody release line on the non-specific protein layer. The labeled antibody can be released from the chromatographic membrane in time when a sample solution flows through the chromatographic membrane, so that reversible adsorption and release of the labeled antibody on the chromatographic membrane can be realized, an antibody binding pad is omitted, and the test precision is improved.

Description

technical field [0001] The invention relates to the field of biomedical in vitro diagnosis, in particular to a test strip based on the release of labeled antibodies on a chromatographic membrane and a preparation method. Background technique [0002] Antibody is a protein that recognizes antigen, and its recognition of antigen has the characteristics of high sensitivity, specificity and strong binding force. Immunochromatography refers to the technology of separating and measuring antigens in samples through specific recognition and binding of antibodies in the process of membrane chromatography, and is often used in the detection of specific antigens in in vitro diagnostics. Test strips in immunochromatography usually contain five components: base plate, sample pad, antibody-binding pad, chromatography membrane, and absorbent pad. Among them, the base plate is used to support the adhesion, the sample pad is used to accept the dripping sample solution, the antibody binding ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/533G01N33/52
CPCG01N33/558G01N33/533G01N33/52
Inventor 赵广宇张伟米朔甫陈金辉
Owner 北京华益精点生物技术有限公司
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