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Method for regenerating plant from panax ginseng isolated microspore embryoid

A technology for regenerating plants and embryoid bodies, which is applied in the field of ginseng free microspore embryoid body regeneration plants, and can solve the problems of lagging microspore culture technology and difficulty in cultivating ginseng free microspores

Pending Publication Date: 2020-11-10
梁江
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the free microspore culture has made good progress in the breeding of Brassica crops (Chinese cabbage, cabbage, rape, etc.), grass crops (wheat, barley, rice), etc., but the ginseng free microspore regeneration system has not yet been established. , which shows that the cultivation of free microspores of ginseng is more difficult, and also shows that its microspore culture technology is lagging behind

Method used

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  • Method for regenerating plant from panax ginseng isolated microspore embryoid
  • Method for regenerating plant from panax ginseng isolated microspore embryoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Test materials: Embryoid bodies of two ginseng varieties "Da Ma Ya" and "Bamboo Ginseng" induced by free microspores have been obtained in the inventor's previous experiments.

[0024] experiment method:

[0025] S1. Seedling cultivation of embryoids: transfer the ginseng free microspore embryoids that have developed to the cotyledon stage into the seedling medium, first induce treatment at a low temperature of 10°C for 10 days, and then at a temperature of 25°C and a light intensity of 1500 lx, The regenerated seedlings were cultivated under the condition of 15 hours of light every day; subcultured once a month during the cultivation process;

[0026] The composition of the seedling medium is: KNO 3 1000mg / L, NH 4 NO 3 600mg / L, K 2 SO 4 170mg / L, KH 2 PO 4 157mg / L, CaCl 2 2H 2 O 310mg / L, MgSO 4 ·7H 2O 195mg / L, FeSO 4 ·7H 2 O 27.8mg / L, Na 2 -EDTA 36.9mg / L, KI 0.85mg / L, H 3 BO 3 6.1mg / L, MnSO 4 4H 2 O 21.8mg / L, ZnSO 4 ·7H 2 O 8.4mg / L, Na 2 MoO 4 2...

Embodiment 2

[0031] Example 2 Effect of low temperature induction treatment on embryoid body seedling formation

[0032] According to the method of Example 1, the ginseng free microspore embryoid body developed to the cotyledon stage was transferred to the seedling medium, induced at a low temperature of 10°C for 0 (CK), 5d, 10d, and 15d, and then at a temperature of 25°C , light intensity 1500lx, culture under the condition of light 15h every day to form regenerated seedlings, count embryoid body seedling rate, the results are shown in Table 1.

[0033]

[0034] It can be seen from Table 1 that the seedling rate of ginseng microspore embryoids was induced by low temperature for 5-15 days compared with the control. Among them, the effect of low temperature treatment for 10 days was the best, and the seedling rate of embryoid bodies of "Da Ma Ya" and "Bamboo Grund" reached 64.7% and 54.8% respectively, which was significantly different from the control, while the effects of low temperatu...

Embodiment 3

[0035] Example 3 Effects of aminooxyacetic acid on embryoid body seedling formation

[0036] According to the method of Example 1, the ginseng free microspore embryoid body developed to the cotyledon stage is transferred to the seedling medium, and the amino acid acetic acid in the medium is set at 5 concentrations of 0, 2.0, 4.0, 6.0 and 8.0mg / L Treatment, other components are unchanged, induction treatment at 10°C low temperature for 10 days, and then at a temperature of 25°C, a light intensity of 1500lx, and 15h of light per day to cultivate and form regenerated seedlings, and count the embryoid body seedling rate, the results are shown in Table 2 Show.

[0037]

[0038] As can be seen from Table 2, compared with the control group, the seedling rate of ginseng microspore embryoid bodies was increased by adding the aminooxyacetic acid of the test concentration, and with the increase of aminooxyacetic acid concentration, the seedling rate showed a higher rate. A trend of ...

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Abstract

The invention provides a method for regenerating a plant from a panax ginseng isolated microspore embryoid. The method comprises the following steps that the isolated microspore embryoid developed toa proper period is transferred into a seedling culture medium, induction treatment ais carried out at a low temperature of 10 DEG C firstly, and then culturing is carried out under a normal illumination condition to form a regenerated seedling; the regenerated seedling is cut and transferred into a rooting culture medium, and continuous illumination culturing is carried out to enable the regenerated seedling to strengthen the seedling and root; and when the regenerated plant grows to 3-5 true leaves and has the robust root, the regenerated plant is placed in a sealed plastic small arched shedto be domesticated, and after survival, the regenerated plant is transplanted into a greenhouse. The invention further provides the embryoid seedling culture medium. The panax ginseng microspore embryoid on the culture medium can well germinate and directly and quickly regenerate the seedling. According to the method, from the aspects of culture conditions, medium components and the like, the influence of a plurality of factors on embryoid development and seedling formation is preliminarily studied, the panax ginseng haploid regenerated plant is successfully obtained, and a foundation is laidfor application in the aspect of isolated microspore culture in panax ginseng breeding.

Description

technical field [0001] The invention relates to a method for regenerating plants from ginseng free microspore embryoid bodies, and belongs to the technical field of traditional Chinese medicine plant cultivation. Background technique [0002] Ginseng ( Panax ginseng C. A. Mey) is a perennial herbaceous plant of the Araliaceae ginseng genus, with a hypertrophic main root, yellowish-white flesh, cylindrical or spindle-shaped, often with branches in the lower part, and long and many fibrous roots. Ginseng usually blooms in 3 years and bears fruit in 5-6 years. The flowering period is July-August and the fruiting period is June-September. Ginseng is afraid of the sun, heat, drought, and cold, and likes a cool, humid climate. It mostly grows in miscellaneous woods or mixed coniferous and broad-leaved forests. Ginseng is mainly distributed in the eastern part of Liaoning, the eastern half of Jilin and the eastern part of Heilongjiang in China. The main producing areas are Fuson...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 梁江
Owner 梁江
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