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A Phellinus strain with high polysaccharide production and its cultivation method and application

A cultivation method and a technique for cultivating mother species are applied to the high-yield polysaccharide Phellinus strain and its cultivation field, which can solve the problems of immature technology, low yield, unstable variety traits, etc., and achieve the effect of genetic stability

Active Publication Date: 2021-12-21
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the particularity and complexity of the physiological state and the constraints of the external environment, Phellinus seldom forms fruiting bodies in nature, especially it takes many years to form usable fruiting bodies, which is far from meeting the needs of the market
However, Phellinus is a kind of white-rot fungus, mainly saprophytic and parasitic, so its artificial cultivation is theoretically feasible. Although artificial cultivation models have appeared in many places in my country, due to immature technology and variety traits Instability and other factors, there are problems such as low yield and poor fruiting body quality

Method used

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  • A Phellinus strain with high polysaccharide production and its cultivation method and application
  • A Phellinus strain with high polysaccharide production and its cultivation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Present embodiment is the identification of poplar phellinus strain S01:

[0023] (1) Morphological identification

[0024] Phellinus fruiting body of poplar Phellinus strain S01, perennial, such as figure 1 as shown, figure 1 The left picture in the middle is the back view, and the right picture is the front view. The edge is a bright yellow growth layer, which gradually lignifies as the fruiting body grows. The cap is horseshoe-shaped, the back is brown, and the belly is yellow. The size is 8.3cm×6.7cm, and the weight is 89.6 g, the hyphae are neat and dense, white at the beginning of germination, and then slowly turn yellow. The growth characteristics of the fruiting body and mycelia described above are basically consistent with the morphological characteristics of Phellinus sangili reported by Wu Shenghua et al. and Dai Yucheng et al.

[0025] (2) Molecular identification

[0026] The total genomic DNA of Phellinus poplar strain S01 was extracted, and the ITS uni...

Embodiment 2

[0029] This embodiment is the cultivation method of the Phellinus poplar strain S01 of Example 1. The Phellinus poplar strain S01 is successively cultivated through the mother species, the original species, the cultivated species and the yellowing stage to obtain a polysaccharide content of 4.36% Phellinus subentity;

[0030] The specific process of cultivating the mother species is as follows: cultivating in dark light for 20 days at a temperature of 25° C.; the medium for cultivating the mother species is made of the following raw materials in parts by weight: 500 parts of mulberry wood chip filtrate, peeled potato filtrate 500 parts, glucose 20 parts, peptone 10 parts, KH 2 PO 4 1 part, 13 parts of agar powder; the mulberry wood chips filtrate is obtained by mixing mulberry wood chips and water with a mass ratio of 1:5, and then filtering at a temperature of 100°C for 30 minutes; the peeled potato filtrate is the mass fraction It is obtained by cutting peeled potatoes at ...

Embodiment 3

[0036] This embodiment is the cultivation method of the Phellinus poplar strain S01 of Example 1. The Phellinus poplar strain S01 is successively cultivated through the mother species, the original species, the cultivated species and the yellowing stage to obtain a polysaccharide content of 4.59% Phellinus subentity;

[0037] The specific process of cultivating the mother species is as follows: cultivating in dark light for 15 days at a temperature of 28° C.; the medium for cultivating the mother species is made of the following raw materials in parts by weight: 600 parts of mulberry wood chip filtrate, peeled potato filtrate 600 parts, 35 parts of glucose, 15 parts of peptone, KH 2 PO 4 2 parts, 15 parts of agar powder; the mulberry wood chip filtrate is obtained by mixing the mulberry wood chip and water with a mass ratio of 1:5, and then filtering it at a temperature of 100°C for 30 minutes; the peeled potato filtrate is the mass fraction It is obtained by cutting peeled ...

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Abstract

The invention provides a Phellinus strain of high yield polysaccharides, the strain number is S01, the preservation number is CGMCC NO.20223, and a cultivation method is provided: the Phellinus strain S01 of poplar is successively cultivated by mother species, original species, cultivated species and The Phellinus Phellinus fruiting bodies are obtained by culturing at the yellowing stage, and the application is also provided for preparing anti-tumor health food or medicine. The poplar phellinus strain S01 of the invention is genetically stable, and the polysaccharide content in the phellinus fruiting body obtained by the strain can reach 4.36%-4.59%. It has strong ability in synthesizing polysaccharides and can be used in the preparation of anti-tumor health food or medicine.

Description

technical field [0001] The invention belongs to the technical field of Phellinus strains, and in particular relates to a high-yield polysaccharide Phellinus strain and its cultivation method and application. Background technique [0002] As a biological response modifier (BRM), polysaccharides have attracted more and more attention from researchers. Because of their natural and non-toxic side effects, polysaccharides may become an ideal source of functional foods and drugs. Studies have found that polysaccharides have a series of effects such as promoting the body's immune function, antibacterial, antiviral, antiparasitic, antitumor and antiradiation, and have been used clinically to treat hepatitis, AIDS and many other diseases. Judging from the statistical data in recent years at home and abroad, tumor diseases have become the world's second "killer" after cardiovascular diseases. Because radiotherapy and chemotherapy treatment methods kill cancer cells, they also produce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/40A01G18/20A61K36/07A61P35/00A23L31/00A23L33/00
CPCA01G18/00A01G18/40A01G18/20A61K36/07A61P35/00A23L31/00A23L33/00A23V2002/00A23V2200/308
Inventor 宋吉玲王伟科袁卫东陆娜闫静
Owner HANGZHOU ACAD OF AGRI SCI
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