Space-mutagenesis lactobacillus reuteri and lactobacillus plantarum compound leavening agent and application thereof to preparation of probiotic yoghourt

A technology of Lactobacillus reuteri and Lactobacillus plantarum, applied in the direction of Lactobacillus, bacteria used in food preparation, applications, etc., can solve the problems of few studies on space-induced mutagenic probiotics and limited bacterial resources, and achieve Fast fermentation speed and good probiotic effect

Pending Publication Date: 2020-11-13
FULLARTON BIOENG TECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited bacterial resources carried in space, there are very few studies on the impact of real space environments on common b

Method used

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  • Space-mutagenesis lactobacillus reuteri and lactobacillus plantarum compound leavening agent and application thereof to preparation of probiotic yoghourt
  • Space-mutagenesis lactobacillus reuteri and lactobacillus plantarum compound leavening agent and application thereof to preparation of probiotic yoghourt
  • Space-mutagenesis lactobacillus reuteri and lactobacillus plantarum compound leavening agent and application thereof to preparation of probiotic yoghourt

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0089] Embodiment 1. Optimization of fermentation process of space mutagenesis probiotic yogurt pilot

[0090] 1. Determination of growth curves of 9-79 and SS18-5 strains and optimization of fermentation conditions

[0091] 1. Determination of growth curves of 9-79 and SS18-5 strains

[0092] The space-mutated Lactobacillus reuteri 9-79 and the space-mutated Lactobacillus plantarum SS18-5 were respectively activated for two generations, inoculated into MRS medium, and cultured at 37°C for 24h. Starting from 0h, samples were taken every 2h to detect the optical density value at 600nm. The culture time was used as the abscissa and the optical density value was used as the ordinate to draw the growth curves of strains 9-79 and SS18-5 respectively.

[0093] The result is as figure 1 shown. Depend on figure 1 (Left) It can be seen that strains 9-79 enter the logarithmic growth phase after 2-6h, and the cell density increases rapidly from 6-18h; the 18h cell density is the highes...

Example Embodiment

[0172] Example 2. Optimization of the pilot-scale fermentation process of space mutagenic probiotic yogurt

[0173] 1. Optimization of pilot fermentation conditions for strains 9-79 and SS18-5

[0174] 1. Activation and expansion of strains

[0175] Take the space-mutated Lactobacillus reuteri 9-79 and the space-mutated Lactobacillus plantarum SS18-5 preserved in glycerol, and activate them into 10 mL MRS medium test tubes. After 2 to 3 passages of activation, expand the culture to 100 mL In a conical flask of MRS medium, culture at 35 to 37 °C for 18 to 22 h, as a 5L fermentor seed, and store at 4 °C for future use.

[0176] 2. Single factor multilevel experiment to determine the fermentation conditions of 9-79 and SS18-5 strains in pilot scale

[0177] (1) Determination of the pH of the fermentation medium: In two 5L fermenters containing 2L MRS medium, the fermentation temperature of Lactobacillus reuteri 9-79 was controlled to be 37°C, the fermentation time was 22h, and ...

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Abstract

The invention discloses a spatial mutagenesis lactobacillus reuteri and lactobacillus plantarum compound leavening agent and application thereof to preparation of probiotic yoghourt. According to theinvention, lactobacillus reuteri 9-79 and lactobacillus plantarum SS18-5 which are carried and mutagenized by a Tiangong II airship and a Shenzhou No.11 airship are used as research objects, the fermentation process conditions of the yogurt and the high density fermentation conditions of the bacterial strains are optimized, the liquid composite fermentation bacterial agent and the dry powder composite fermentation bacterial agent are developed, and the probiotic yogurt with characteristics of rapid fermentation, good probiotic effect and unique fried wheat aroma is developed by using the bacterial agents. After space environment mutagenesis, space mutagenesis strains 9-79 and SS18-5 with excellent fermentation performance are bred to serve as a composite fermentation inoculant to produce the probiotic yoghourt, the blank of domestic space probiotic yoghourt composite leavening agents is filled up, and technical support is provided for industrial application of research and developmentachievements of space probiotic yoghourt products.

Description

technical field [0001] The invention belongs to the technical field of microbes and food processing, and in particular relates to a space-mutated Lactobacillus reuteri and Lactobacillus plantarum composite starter and its application in the preparation of probiotic yoghurt. Background technique [0002] As a nutritious and healthy food, yogurt has long been popular all over the world, and even occupies more than 50% of the liquid milk market in some developed countries and regions. With the improvement of the living standard of Chinese residents and the enhancement of health awareness, the status of dairy products in the dietary structure of residents has been significantly improved. As a food with health functions, yogurt is loved by consumers. In recent years, the growth rate of my country's yogurt market has led the world, maintaining double-digit growth every year. In 2016, the market size of China's yogurt industry reached 101.017 billion yuan, the market exceeded 100 ...

Claims

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Application Information

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IPC IPC(8): A23C9/123A23C9/13C12N1/20C12R1/225C12R1/25
CPCA23C9/1234A23C9/1307C12N1/20A23V2400/173A23V2400/169
Inventor 郝红炜袁明刘慧张红星谢远红
Owner FULLARTON BIOENG TECH BEIJING CO LTD
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