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Repeated application production method for low-temperature bio-enzyme catalyzed deodorization distillate esterification reaction

A technology of deodorization distillate and esterification reaction, applied in the direction of fermentation, etc., can solve the problems of limiting the use of biological enzymes and high production costs, and achieve the effects of ensuring the esterification rate, improving the use effect, and simple process flow

Pending Publication Date: 2020-11-13
FUJIAN GLANNY BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production cost of biological enzymes is relatively high compared with traditional acid-base catalysts, which limits the application of biological enzymes in industrial esterification reactions

Method used

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  • Repeated application production method for low-temperature bio-enzyme catalyzed deodorization distillate esterification reaction
  • Repeated application production method for low-temperature bio-enzyme catalyzed deodorization distillate esterification reaction
  • Repeated application production method for low-temperature bio-enzyme catalyzed deodorization distillate esterification reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) The quality of DD oil is 200kg, the amount of biological enzyme is 8‰ of the quality of DD oil, the quality of water is 3% of the quality of DD oil, the quality of alkali solution is 0.12% of the quality of DD oil, and the quality of methanol is 10% of the quality of DD oil. The mass of liquid A is 5% of the mass of DD oil, the esterification temperature: 40°C, and the esterification time: 12h. Among them, ET2.0 is selected as the biological enzyme, and the alkali solution is potassium hydroxide solution with a concentration of 16%. The mixed solution A is a mixed solution formed by mixing two or more of methanol, ethanol, methyl ester, sodium phosphate, water, ethyl acetate, etc.

[0026] 2) The first esterification rate is 95.8%. Esterification rate=100%*(initial acid value-final acid value) / initial acid value.

[0027] 3) Filter the esterification liquid and recover the biological enzyme through the enzyme repeated application device; carry out the next esterifi...

Embodiment 2

[0042] 1) The quality of DD oil is 200kg, the amount of biological enzyme is 1.5‰ of the quality of DD oil, the quality of water is 5‰ of the quality of DD oil, the quality of alkali solution is 0.15% of the quality of DD oil, and the quality of methanol is 12% of the quality of DD oil. The mass of liquid A is 3% of the mass of DD oil, the esterification temperature: 35°C, and the esterification time: 14h. Among them, ET2.0 is selected as the biological enzyme, and the alkali solution is potassium hydroxide solution with a concentration of 16%. The mixed solution A is a mixed solution formed by mixing two or more of methanol, ethanol, methyl ester, sodium phosphate, water, ethyl acetate, etc.

[0043] 2) The first esterification rate is 95.4%.

[0044] 3) Filter the esterification liquid and recover the biological enzyme through the enzyme repeated application device; carry out the next esterification of the recovered biological enzyme, and perform the esterification reaction...

Embodiment 3

[0049] 1) The quality of DD oil is 200kg, the amount of biological enzyme is 3% of the quality of DD oil, the quality of water is 1% of the quality of DD oil, the quality of alkaline solution is 0.2% of the quality of DD oil, and the quality of methanol is 15% of the quality of DD oil. The mass of liquid A is 0.5% of the mass of DD oil, the esterification temperature: 25°C, and the esterification time: 18h. Among them, ET2.0 is selected as the biological enzyme, and the alkali solution is potassium hydroxide solution with a concentration of 16%. The mixed solution A is a mixed solution formed by mixing two or more of methanol, ethanol, methyl ester, sodium phosphate, water, ethyl acetate, etc.

[0050] 2) The first esterification rate is 95.6%.

[0051] 3) Filter the esterification liquid and recover the biological enzyme through the enzyme repeated application device; carry out the next esterification of the recovered biological enzyme, and perform the esterification reactio...

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Abstract

The invention relates to the technical field of biochemical engineering, in particular to a repeated application production method for low-temperature bio-enzyme catalyzed deodorization distillate esterification reaction. The method comprises the following steps: (1), proportionally adding DD oil, bio-enzyme, water, an alkaline solution, methanol and a mixed solution A into a reaction kettle, andcarrying out esterification reaction at 25-40 DEG C for 12-24 hours; (2), after the esterification reaction is finished, repeatedly applying the device with an enzyme, filtering esterification liquid,and recycling a biological enzyme; (3), carrying out next esterification on the recovered biological enzyme, executing the feeding quality and reaction conditions of DD oil, water, an alkaline solution, methanol and the mixed solution A according to the step (1); and (4) circulating the steps (2) and (3), and supplementing biological enzyme which accounts for 5-15% of the initial enzyme amount after every three times of esterification reaction. The repeated application process provided by the invention is simple in flow, the biological enzyme can be effectively recycled and repeatedly utilized, the utilization rate of the biological enzyme is improved, the use effect of the biological enzyme can be improved by adding the mixed solution A, the production cost is reduced, and the method issuitable for popularization and application.

Description

technical field [0001] The invention relates to the technical field of biochemical industry, in particular to a repeatedly applied production method for the esterification reaction of deodorized distillates catalyzed by low-temperature biological enzymes. Background technique [0002] Bioenzyme esterification extracts phytosterols in vegetable oil deodorization distillate (DD oil), which has the advantages of mild operating conditions, strong selectivity, less damage to active substances, and environmental protection. It is a high resource utilization of vegetable oil deodorization distillate The development direction of value utilization. However, compared with traditional acid-base catalysts, the production cost of biological enzymes is relatively high, which limits the application of biological enzymes in industrial esterification reactions. Contents of the invention [0003] In view of the above problems, the present invention provides a repeatedly applied production ...

Claims

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Application Information

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IPC IPC(8): C12P7/62
CPCC12P7/62
Inventor 邱建国梁玉龙翁庆水林宜明谢杰华
Owner FUJIAN GLANNY BIO ENG