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Tumor microsatellite instability detection kit based on second-generation sequencing probe capture technology

A technology of microsatellite instability and detection kit, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., to achieve the effect of wide range of tumors, good specificity and high detection accuracy

Pending Publication Date: 2020-11-13
俊兮生物科技(上海)有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, MMR-IHC has limitations. In MSI-H, 5% of the tumors express the above four MMR proteins.

Method used

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  • Tumor microsatellite instability detection kit based on second-generation sequencing probe capture technology
  • Tumor microsatellite instability detection kit based on second-generation sequencing probe capture technology
  • Tumor microsatellite instability detection kit based on second-generation sequencing probe capture technology

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Embodiment Construction

[0071] The technical solutions of the present invention will be described below in conjunction with the embodiments.

[0072] The tumor microsatellite instability detection kit based on the second-generation sequencing probe capture technology of the present invention, the detection kit adopts the second-generation sequencing probe capture technology, and the reagents of the detection kit include quality control products, library building reagents and RNA probes;

[0073] The quality control product includes a negative quality control product and a positive quality control product; the negative quality control product is a negative sample dissolved in TE, and the positive quality control product is a plasmid containing an MSI site dissolved in TE;

[0074] The reagents for building a library include end repair & A reagents, adapter reagents, amplification reagents, hybridization reagents, capture reagents and amplification reagents;

[0075] The RNA probe is an RNA single-str...

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Abstract

The invention provides a tumor microsatellite instability detection kit based on a second-generation sequencing probe capture technology. The detection kit adopts the second-generation sequencing probe capture technology, and reagents of the detection kit comprise a quality control product, a library building reagent and an RNA probe; the quality control products comprise a negative quality control product and a positive quality control product; the negative quality control product is a negative sample dissolved in TE, and the positive quality control product is a plasmid containing an MSI site dissolved in TE; an NGS technology is selected on the basis of an MSI experimental method; the kit has the advantages that detection sites are many, up to 40 microsatellite sites are covered after screening, the microsatellite coverage area is wide, the uniformity is good after RNA probe adjustment, good medication guidance can be provided for patients, and the kit is suitable for multiple tumors such as colorectal cancer, endometrial cancer and gastrointestinal tumor.

Description

technical field [0001] The invention relates to the technical field of detection kits, in particular to a detection kit for tumor microsatellite instability based on the second-generation sequencing probe capture technology. Background technique [0002] Microsatellite (Microsatellite) refers to a short tandem repeat sequence usually with 1-6 bp as a repeating unit and the number of repeats ≤ 60 times. Microsatellite loci are scattered on each chromosome, and they are mutation-prone regions, showing a high degree of polymorphism, specifically manifested as duplication of microsatellite locus repeating units (1-6bp) between different individuals or between normal tissues and tumor tissues Times are volatile. Based on the widespread distribution and high polymorphism of microsatellite loci, microsatellites are often used to study the mechanism of tumorigenesis. [0003] Microsatellite instability (Microsatellite Instability, MSI) refers to the "strand slip" error in the DNA ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6869
CPCC12Q1/6886C12Q1/6869C12Q2600/156C12Q2600/106C12Q2523/301C12Q2525/191C12Q2525/173C12Q2563/143C12Q2563/149C12Q2535/122
Inventor 方小云
Owner 俊兮生物科技(上海)有限公司
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