Intergeneric distant hybrid creation method of crossostephium chinense and creeping ground-cover chrysanthemum

A technology of distant hybrid and hibiscus chrysanthemum, which is applied in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, can solve the problems of small immature embryos, few applications, and difficulty in peeling off, and achieves the effect of avoiding premature senescence of embryos.

Active Publication Date: 2020-11-17
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Embryo rescue, as an important means to overcome the barriers of distant hybridization, has been applied in Chrysanthemum, but because Hibiscus h

Method used

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  • Intergeneric distant hybrid creation method of crossostephium chinense and creeping ground-cover chrysanthemum
  • Intergeneric distant hybrid creation method of crossostephium chinense and creeping ground-cover chrysanthemum
  • Intergeneric distant hybrid creation method of crossostephium chinense and creeping ground-cover chrysanthemum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Embryo rescue using young embryos of different embryonic ages to obtain intergeneric hybrid offspring of hibiscus and creeper chrysanthemum

[0065] Embryo rescue using young embryos of different embryonic ages to obtain intergeneric hybrid offspring of hibiscus chrysanthemum and creeper chrysanthemum comprises the following steps:

[0066] 1. Preparation of parents: the female parent selects the diploid salt-tolerant wild species Cr.chinense of the genus Chrysanthemum (Cr. Adjust the flowering period of Hibiscus chrysanthemum, put it in the artificial climate chamber for 8 hours of short-day maintenance in August, and reach the full flowering period in mid-October; the short-day artificial climate chamber is set at 70% humidity, 25°C during the day and 22°C at night , the light time is from 6 o'clock to 14 o'clock, and the light intensity is 1600-2000lx.

[0067] 2. Distant hybridization: In the Xiaotangshan Nursery of the National Flower Engineering Center,...

Embodiment 2

[0074] Example 2 Using media with different hormone ratios for embryo rescue to obtain intergeneric hybrid offspring of hibiscus and creeper chrysanthemum

[0075] Embryo rescue using medium with different hormone ratios to obtain intergenus hybrid offspring of hibiscus and creeper chrysanthemum, including the following steps:

[0076] 1. Preparation of parents: the female parent is selected from the diploid salt-tolerant wild species of Furong Chrysanthemum (Cr. Adjust the flowering period of Hibiscus chrysanthemum, put it in the artificial climate chamber for 8 hours of short-day maintenance in August, and reach the full flowering period in mid-October; the short-day artificial climate chamber is set at 70% humidity, 25°C during the day and 22°C at night , the light time is 6:00-14:00, and the light intensity is 1600-2000lx.

[0077] For the comparison between the female parent Hibiscus chrysanthemum and the male parent Creeperia chrysanthemum, see figure 1 .

[0078] 2. ...

Embodiment 3

[0086] Example 3 Using Morphological Observation, Chromosome Counting and Flow Cytometry to Identify the Authenticity of Intergenus Hybrids of Hibiscus and Creeperia

[0087] Using morphological observation, chromosome counting and flow cytometry analysis to identify the authenticity of intergeneric hybrids of hibiscus and creeper, including the following steps:

[0088] 1. Identification of Hybrid Authenticity Using Morphology

[0089] 1) The parents and hybrid offspring were planted in the open field in May for routine maintenance and management. In autumn, observe and count the morphology of the parents and offspring materials, including: measuring plant height, crown width, and leaf traits.

[0090] 2) Each plant randomly selects three fully expanded leaves, measures the leaf length and leaf width, and measures the length of the petiole respectively; wherein the plant height, crown width, leaf length, leaf width, and petiole length are between the parents. identified as ...

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Abstract

The invention discloses an intergeneric distant hybrid creation method of crossostephium chinense and creeping ground-cover chrysanthemum. The method includes the steps that artificial cross breedingis carried out by taking crossostephium chinense of diploid salt-tolerant species in crossostephium chinense genera of compositae as a female parent and creeping ground-cover chrysanthemum of tetraploid salt-tolerant species in chrysanthemum genera of the compositae as a male parent; F1-generation plants are obtained through cross pollination and immature embryo in-vitro culture; and the F1-generation material is subjected to hybrid morphological observation, cytological identification, and flow cytometry analysis to finally obtain intergeneric true hybrid progenies of the crossostephium chinense and the creeping ground-cover chrysanthemum, so that the intergeneric distant hybridization obstacle of the crossostephium chinense and the creeping ground-cover chrysanthemum is successfully overcome. The hybrid survival rate is increased, the chrysanthemum cultivation period is shortened, and a batch of new germplasm is created.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for creating intergenus distant hybrids of hibiscus chrysanthemum and creeper chrysanthemum. Background technique [0002] Hibiscus chrysanthemum (Crossostephium chinense), a single genus and single species of Asteraceae, is unique to my country and is produced in the southeast coastal area. Evergreen subshrub. The leaves are often narrowly oblanceolate, often entire, densely covered with gray pubescence on both sides, and thick in texture; the flower diameter is about 0.5cm, light yellow, without tongue-shaped flowers, blooming from late November to January of the next year. Hibiscus chrysanthemum is a rare natural plant with different color leaves and aromatic plants. It also has the characteristics of salt and alkali resistance, wind resistance and less pests and diseases, so it is widely used in gardens. Yang Haiyan (2016) used 8 wild species of Chrysanthemum genus as...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/008A01H4/001
Inventor 孙明许婷陈俊通钟剑郭彦宏李大伟巴婷婷常丽娜张启翔程堂仁
Owner BEIJING FORESTRY UNIVERSITY
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