A kind of compound microbial bacterial agent and its application in regulating pit mud ph

A technology of composite microbial agent and composite microbial bacteria, which is applied in the fields of fermentation and biology, can solve problems such as interference, achieve the effects of reducing accumulation, preventing the formation of calcified substances, and reducing redox potential

Active Publication Date: 2022-05-20
JIANGSU YANGHE BREWERY JOINT STOCK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the pit mud micro-ecosystem has self-healing functions, the optimization of human interference, maintenance of pit mud with sufficient water, balanced nutrition, and appropriate pH value and other pit maintenance measures have a great impact on the construction and maintenance of a good micro-ecological flora.
Currently, chemical addition of Ca(OH) is used in pit mud production 2 , ammonia, etc. to adjust the pH of the artificial pit mud, which did not fundamentally solve the acidification problem of the pit mud

Method used

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  • A kind of compound microbial bacterial agent and its application in regulating pit mud ph
  • A kind of compound microbial bacterial agent and its application in regulating pit mud ph
  • A kind of compound microbial bacterial agent and its application in regulating pit mud ph

Examples

Experimental program
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preparation example Construction

[0050] The preparation method of the seed solution involved in the following embodiment is as follows:

[0051] Preparation method of lactic acid using bacterial seed liquid: Bacillus cereus CCTCC AB 205244 and single colonies of Bacillus subspercuss subspermia subspermia C. subspermia CICC 20855 were inoculated into sodium lactate liquid medium, and the high liquid was incubated for 5-7 days at 37 °C, and the seed solution of Bacillus cereus CCTCC AB 205244 and the seed solution of Bacillus subspercuss subspersion CICC 20855 were obtained, respectively.

[0052] Preparation method of acetic acid using bacterial seed liquid: Bacillus cereus CCTCC AB 205244, Bacillus myristilines CGMCC 1.3475, Bacillus lysine CICC 20021, Bacillus australis soil CCTCC KB20081573 were inoculated into sodium acetate liquid medium, and the high liquid was incubated at 37 °C for 5 days to obtain Bacillus cereus CCTCC AB 205244 seed solution, respectively. Bacillus myristemis lysergic CGMCC 1.3475 seed s...

Embodiment 1

[0056] Example 1: Preparation of a compound microbial agent

[0057] The specific steps are as follows:

[0058] 1. Preparation of compound bacterial liquid,

[0059] (1) Preparation of compound lactic acid using bacterial liquid: Bacillus cereus CCTCC AB 205244 and Bacillus subspercuss subspermia CICC 20855 were simultaneously inoculated into sodium lactate liquid medium according to the inoculation amount of 1:1 of the bacterial volume ratio, and the high liquid at 37 °C was incubated for 5-7 days to the number of microscopic bodies 2.0×10 10 / mL, to obtain a compound lactic acid using bacterial liquid; wherein, the Bacillus cereus CCTCC AB 205244, Bacillus subspermia subspermia CICC 20855 in the form of seed solution inoculated into sodium lactate liquid medium, and, Bacillus cereus CCTCC AB 205244 seed solution and Bacillus subspercuss ceresia subspecies CICC 20855 seed liquid in the medium the total inoculation amount of 10% (v / v).

[0060](2) Preparation of compound aceti...

Embodiment 2

[0070] Example 2: Application of compound microbial agent in regulating the pH of cellar mud

[0071] The specific steps are as follows:

[0072](1) Pit punching: Use the punching fork to punch the bottom of the cellar and the cellar wall, the diagonal fork out of the radius of 1.5cm, the depth of 4cm of the small hole, 30 small holes per square meter, near the upper part of the cellar pool 50-60cm, the density of the piercing eye density becomes larger, 50 small holes per square meter.

[0073] (2) After the hole is laid, from the upper part of the cellar pool down, into the hole into the hole in the compound microbial agent obtained in Example 1, each pool with a compound microbial agent 20kg, the cellar pool length of 3.4m, width 2.1m, depth of 1.65m, cellar area of 25m 2 。

[0074] (3) Maintenance: after adding cellar mud to the cellar pool, smoothing the holes, limited to the maintenance before the summer cellar pressing, the temperature is set at 20-25 ° C, after maintenance...

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Abstract

The invention relates to a compound microbial bacterial agent and its application in regulating the pH of pit mud, and belongs to the field of biotechnology and fermentation technology. The invention provides a composite microbial bacterial agent, which includes composite microorganisms and yellow water; the composite microorganisms include composite acetic acid-utilizing bacteria, composite lactic acid-utilizing bacteria, composite nitrate-reducing bacteria, composite actinomycetes and Composite sulfate-reducing bacteria, the method of the present invention follows the principle of cellar micro-ecology, selects various microorganisms such as acetic acid-utilizing bacteria, reducing bacteria, actinomycetes, and lactic acid-utilizing bacteria, and uses them in combination to construct a benign cellar micro-ecology. The provided compound microbial agent can regulate the pH in the pit mud, maintain the pH of the pit mud at an optimal state of 6.0-6.8, alleviate the aging of the pit mud, and stabilize the micro-ecological structure in the pit mud.

Description

Technical field [0001] The present invention relates to a compound microbial agent and its application in regulating the pH of the cellar mud, belonging to the field of biotechnology and fermentation technology. Background [0002] "Thousands of years old cellar is bad, the wine is good all by the cellar pool old" high-quality cellar mud can significantly improve the quality of aromatic liquor base wine. However, in the process of natural aging, there are a small number of cellar ponds will degenerate, high-quality cellar mud compared with degraded cellar mud, the most intuitive performance is that the moisture of degraded cellar mud, pH value is significantly lower than that of high-quality cellar mud (pH 4.1-5.6), the cellar mud appears plated and hardened, the surface of the cellar mud is mixed with a large number of calcium lactate, iron lactate crystallization, beneficial bacteria in the aging cellar mud, caproic acid bacteria, butyric acid bacteria, yeast and other metaboli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12G3/02C12R1/085C12R1/01C12R1/125C12R1/145C12R1/47
CPCC12N1/20C12G3/02Y02W10/20
Inventor 谢旭苗秀珍刘乐乐吴琼张龙云朱超朱青
Owner JIANGSU YANGHE BREWERY JOINT STOCK
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