Joint detection test paper for ten respiratory tract pathogens and preparation method thereof

Abstract

The invention discloses joint detection test paper for ten respiratory tract pathogens and a preparation method of the joint detection test paper. The paper comprises a first nitrocellulose layer, a waterproof double-sided adhesive tape, a second nitrocellulose layer and a PVC bottom plate which are sequentially stacked from top to bottom. The paper can be used for rapidly detecting 10 pathogens on one piece of paper at the same time; through the two sample dripping ports, simultaneous detection of 10 detection lines can be realized, pollution caused by frequent operation is avoided, the consumption of samples is greatly reduced, and the accuracy of detection results is improved; by adjusting the antibody coated by the test paper, multiple types of pathogens can be simultaneously detectedor the same pathogen can be detected in parallel by one piece of test paper; the detection line and the quality control line are positioned on the same horizontal axis / longitudinal axis, so that detection results can be conveniently compared. Compared with other test paper, the kit is more efficient and accurate, and can provide technical support for on-site rapid detection of pathogens.

Description

Technical field [0001] The present invention relates to the field of pathogen detection, particularly to a joint detection of respiratory pathogens ten kinds of paper and its preparation method. Background technique [0002] Immunological detection techniques and nucleic acid detection technology is an important means to detect the current large-scale novel coronavirus (2019-nCoV), respiratory disease. Wherein the real-time PCR technique (QuantitativeReal-time PCR, RT-PCR) of nucleic acid detection method is mainly used in clinical detection. This technique is a fluorescent group in the PCR reaction system, since the fluorophore will bind to the DNA so that the fluorescence intensity changes. Thus real-time collection of the fluorescence signal in the course of the reaction, it can be tested samples. The good method sensitivity and specificity. But also has some limitations, such as (1) instruments and equipment requirements (RT-PCR instrument) higher (2) complex operation, long ...

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Problems solved by technology

But it also has some limitations, such as (1) high requirements for instruments and equipment (RT-PCR instrument) (2) complex operation and long operation time (3) high requirements for primers, prone to non-specific bands (4) Infectious materials or live viruses need to be inactivated for nucleic acid testing, which is prone to false negatives

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