Sesamum indicum unpollinated ovary in-vitro culture callus induction method

A technology of callus induction and in vitro culture, applied in horticultural methods, botany equipment and methods, horticulture, etc., to achieve the effects of improving induction efficiency, promoting the formation of callus, and increasing the rate of callus induction

Inactive Publication Date: 2020-11-24
江苏春之雨生物科技发展有限公司
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Problems solved by technology

So far, there is no report on the haploid induced by in vitro culture of unfertilized gynonuclei in sesame

Method used

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  • Sesamum indicum unpollinated ovary in-vitro culture callus induction method
  • Sesamum indicum unpollinated ovary in-vitro culture callus induction method
  • Sesamum indicum unpollinated ovary in-vitro culture callus induction method

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Embodiment 1

[0022] 1 Materials and methods

[0023] 1.1 Test material

[0024] The test materials used in this study were sesame cultivars 'Yuzhi 4' and 'Zheng Heizhi 1'.

[0025] 1.2 Test method

[0026] (1) Material selection and disinfection: select sesame plants that grow robustly and are free from diseases and insect pests, and select non-pollinated flower buds with uncracked anthers 1-2 days before flowering; disinfect the flower buds: first disinfect with 75% alcohol for 30 seconds, and rinse with sterile water Once, disinfect with 3% sodium hypochlorite for 8 minutes, rinse with sterile water 4 to 5 times, and dry the surface with sterile filter paper.

[0027] (2) Pretreatment of explants: the ovary was stripped from flower buds under aseptic conditions as explants, and soaked in a certain concentration of vitamin C solution for 2 hours.

[0028] (3) Explant inoculation and induction culture: Inoculate the explants flat on the induction medium, first perform heat shock treatme...

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Abstract

The invention provides a sesamum indicum unpollinated ovary in-vitro culture callus induction method. The method comprises the following steps: (1) material selection and disinfection: selecting a sesamum indicum plant which grows robustly and has no plant diseases and insect pests, collecting unpollinated flower buds of which anthers do not crack 1-2 days before flowering, and carrying out disinfection treatment; (2) explant pretreatment: stripping ovaries as explants, and soaking the explants in a vitamin C solution with a certain concentration for 2 hours; and (3) explant inoculation and induction culture: flatly placing and inoculating the explant on an induction culture medium, carrying out heat shock treatment for 24-48 hours, then carrying out induction culture at the culture temperature of 23-28 DEG C, carrying out dark culture for the first 30 days in the culture period, and then carrying out illumination culture. According to the method disclosed by the invention, the sesamumindicum unpollinated ovary callus is successfully induced, and the highest induction rate can reach 36.74%. The invention lays a material foundation for further establishing a complete sesamum indicum unpollinated ovary in-vitro culture system.

Description

technical field [0001] The invention relates to a callus induction method for in vitro culture of non-pollinated ovary of sesame, which belongs to the technical field of modern crop breeding. Background technique [0002] Sesame( Sesamum indicum L.) belongs to the genus Sesamum of the family Pedaliaceae. It is one of the oldest oil crops in the world. It has a cultivation history of more than 2,000 years. It is mainly distributed in Asia, Africa and other countries, in India, Sudan, Myanmar and my country. The planting area is relatively large, and the output per unit area and total output are the largest in my country. Sesame is widely planted in my country, mainly distributed in the middle and lower reaches of the Yellow River and the Yangtze River, as well as in Henan, Hubei, Anhui, Jiangxi, Hebei and other provinces. Among them, Henan produces the most, accounting for about 30% of the country's total. According to the color of the seed coat, sesame can be divided into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001A01H4/00
Inventor 任西华张小林
Owner 江苏春之雨生物科技发展有限公司
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