Aerobic cell culture device capable of automatically delivering oxygen

Abstract

The invention relates to the technical field of cell culture, and discloses an aerobic cell culture device capable of automatically delivering oxygen. The aerobic cell culture device comprises a culture device main body, a fixed plate and a driving device, wherein a culture chamber is arranged at the bottom of the culture device main body, a detection box is fixedly arranged at the right side of the culture chamber, a baffle is fixedly arranged in the detection box, and a first air bag is arranged below the baffle; and a second air bag is arranged above an extrusion part, and an oxygen delivering pipe is fixedly connected to the upper portion of the second air bag. According to the aerobic cell culture device capable of automatically delivering the oxygen, when the oxygen is consumed, theupper portion of the extrusion part extrudes the second air bag, and the oxygen in the second air bag jacks up a sealing ball through the oxygen delivering pipe and enters the culture chamber, so thatthe oxygen in the culture chamber is conveyed; and the first air bag is bulged again to the initial state along with the increase of oxygen content in the culture chamber, the driving device stops moving, and oxygen delivering is finished, so that the effects of automatically delivering oxygen when oxygen is consumed and automatically stopping delivering oxygen after oxygen delivery is finished are achieved.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an aerobic cell culture device for automatically delivering oxygen. Background technique [0002] Biological cell culture refers to a method of simulating the internal environment in vitro, so that it can survive, grow, reproduce and maintain the main structure and function, in which aerobic cells need a lot of oxygen during the growth process, and cell culture needs to avoid as much as possible The interference of external factors, the current process of aerobic cell culture requires the input of oxygen for the normal growth of cells, and the frequent opening of the medium to input oxygen will easily interfere with the cell culture process by external bacteria, and it cannot be based on the oxygen consumption in the medium. Oxygen delivery is easy to cause too much oxygen to cause cell death, and manual delivery of oxygen consumes manpower and time. In order to solve the ab...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the invention provides an aerobic cell culture device that automatically delivers oxygen, which can automatically input oxygen according to the oxygen consumption in the culture medium, and when the oxygen is input, the oxygen can be fully dissolved in the culture medium. The advantage of automatic stop of the device solves the problem that frequent opening of the culture medium to input oxygen will easily cause the cell culture process to be disturbed by external bacteria, and the oxygen supply cannot be carried out according to the oxygen consumption in the culture medium, which will easily cause too much oxygen to cause cell death

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