Method for detecting three markers of vitamin B1 in small amount of whole blood sample

Abstract

The invention relates to a simple and reliable method for detecting three markers of vitamin B1 in a small amount of whole blood sample. The method comprises the following steps of I, preserving the sample, II, removing protein and extracting a target compound in sample pretreatment, III, conducting a derivatization step in sample pretreatmentI, IV, adjusting the pH value of the sample before thesample is loaded, V, conducting analysis and quantification of a high performance liquid chromatography (UPLC), wherein the sample is a whole blood sample stored in an anticoagulation tube. The methodcan use a blood sample subjected to EDTA or heparin anticoagulation treatment, is used for determining vitamin B1 nutritional ingredients for crowd analysis and clinical tests, and has the advantagesof low sample use amount, simplicity, sensitivity, strong specificity, good repeatability, good stability and low required cost.

Description

technical field [0001] The invention belongs to the technical field of blood detection methods, and in particular relates to a method for detecting three markers of vitamin B1 in a small amount of whole blood samples. Background technique [0002] As a water-soluble vitamin, vitamin B1, also known as thiamine, plays an important role in energy production and metabolism as an essential nutrient. Vitamin B1 deficiency commonly causes beriberi, optic neuropathy, and other related diseases that increase infant mortality. Therefore, an accurate understanding of vitamin B1 levels in high-risk groups (such as Southeast Asia and other underdeveloped regions, including China) is of great significance for population surveys and clinical trials. Given that qualitative studies such as population surveys often involve a large number of cohort samples with limited blood volume, how to accurately detect vitamin B1 from a small number of samples at a lower cost has important practical sign...

AI Technical Summary

Problems solved by technology

However, according to the research of the inventor, it is found that in the patents that have been declared in China, the detection of B1 level is mainly focused on thiamine Th in the marker (accounting for <5% of the total B1), so it is impossible to accurately evaluate the true level of B1 in patients. Level

However, in the reports on the detection method of vitamin B1 in the English scientific research literature, the reduction of the amount of samples, the cost of the method and whether the method can be used for long-term stable and reliable detection are generally ignored.

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