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Method for promoting macrophages to polarize to M1 type by utilizing piezoelectric effect and application

A macrophage and piezoelectric effect technology, applied in biochemical equipment and methods, animal cells, tumors/cancer cells, etc., can solve problems such as infection, achieve the effect of promoting, reducing infection and rejection

Active Publication Date: 2020-12-04
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But they require invasive wires to connect to complex external devices before being implanted in the body, which not only brings great inconvenience to patients, but also may pose risks such as infection

Method used

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  • Method for promoting macrophages to polarize to M1 type by utilizing piezoelectric effect and application
  • Method for promoting macrophages to polarize to M1 type by utilizing piezoelectric effect and application
  • Method for promoting macrophages to polarize to M1 type by utilizing piezoelectric effect and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The morphology of the β-phase PVDF piezoelectric film polarized along the d33 direction was observed by scanning electron microscope. The scanning electron microscope photos are as follows: figure 1 As shown, the surface of the film is smooth and flat; the piezoelectric response of the β-phase PVDF film was verified by piezoelectric force microscopy, and the results are as follows figure 2 As shown, the butterfly curve represents the piezoelectric response of the material.

[0042] The above-mentioned piezoelectric film is used as a cell culture substrate to promote the polarization of macrophages to the M1 type, and the steps are as follows:

[0043] 1) After soaking and cleaning the β-phase PVDF piezoelectric film fully with deionized water, cut it into 1×1cm 2 , a small piece with a thickness of 100 μm is used;

[0044] 2) Place the β-phase PVDF piezoelectric film in the cell culture well plate, add 75% alcohol to soak for 2 hours, and at the same time, irradiate ...

Embodiment 2

[0049] The β-phase PVDF piezoelectric film is used as a cell culture substrate to promote the polarization of macrophages to the M1 type, and the steps are as follows:

[0050] 1) After the β-phase PVDF piezoelectric film is fully soaked and cleaned with deionized water, it is cut into 1.5×1.5cm 2 , a small piece with a thickness of 200 μm is ready for use;

[0051] 2) Place the β-phase PVDF piezoelectric film in the cell culture well plate, add 75% alcohol to soak for 2 hours, and at the same time, irradiate with ultraviolet light for 60 minutes in a sterile operating table for sterilization, and use PBS buffer solution to clean the residual alcohol; Soak the β-phase PVDF piezoelectric film in high-sugar DMEM medium overnight, and then wash it with PBS buffer 2-3 times for later use;

[0052] 3) Digest the RAW264.7 cells and disperse them with high-sugar DMEM medium to obtain a cell suspension. Inoculate the RAW264.7 cells on the piezoelectric film treated in step 1), and th...

Embodiment 3

[0056] The β-phase PVDF piezoelectric film is used as a cell culture substrate to promote the polarization of macrophages to the M1 type, and the steps are as follows:

[0057] 1) After soaking and cleaning the β-phase PVDF piezoelectric film fully with deionized water, cut it into 1×1cm 2 , a small piece with a thickness of 500 μm is used;

[0058] 2) Place the β-phase PVDF piezoelectric film in the cell culture well plate, add 75% alcohol to soak for 2 hours, and at the same time, irradiate with ultraviolet light for 60 minutes in a sterile operating table for sterilization, and use PBS buffer solution to clean the residual alcohol; Soak the β-phase PVDF piezoelectric film in 1640 medium overnight, then wash it with PBS buffer 2-3 times for later use;

[0059] 3) The primary mouse macrophages were digested and dispersed with 1640 medium to obtain a cell suspension, and the primary mouse macrophages were inoculated on the piezoelectric film after step 1), and the inoculated ...

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Abstract

The invention relates to a method for promoting macrophages to polarize to M1 type by utilizing a piezoelectric effect and application. According to the method, a piezoelectric material is combined with ultrasound to trigger non-contact and non-invasive electrical stimulation, and the important role of the electrical stimulation in immune regulation is verified; by taking the piezoelectric material as a cell culture substrate, and combining with ultrasound stimulation, M1 type polarized macrophages are cultured in vitro, and compared with a common biochemical method, the method does not need to introduce external polarized macrophages (PMAPs); and by utilizing the commercialized piezoelectric material, macrophages can be rapidly promoted to polarize to the M1 type by a method for applyingthe ultrasound; The method has a good effect of promoting the macrophages to polarize to the M1 type by utilizing the piezoelectric effect, and has the clinical application potential of tumor immunotherapy and anti-infective therapy. The method disclosed by the invention can be used for producing the M1 type polarized macrophages in batches so as to promote application of the piezoelectric material in immunotherapy.

Description

technical field [0001] The invention relates to a method and application for promoting the polarization of macrophages to M1 type by using piezoelectric effect, and belongs to the technical field of biomaterials. Background technique [0002] During inflammation, immune homeostasis can be significantly altered by the influence of microbial danger signals, which are collectively referred to as pathogen-associated molecular patterns (PAMPs), including microorganisms, lipopolysaccharide (LPS), DNA, RNA Wait. In the inflammatory microenvironment, PAMPs will guide the immune process, and immune cells can not only respond to PAMPs through pattern recognition receptors, but also coordinate or individually through various microenvironmental receptors that sense temperature, pH, osmotic pressure, oxygen, etc. Regulate immune cells. Macrophages are the most numerous immune cells in the tumor microenvironment and play a significant role in the occurrence and development of tumors. Ma...

Claims

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Application Information

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IPC IPC(8): C12N5/0786C12N5/09C12N13/00
CPCC12N5/0645C12N5/0694C12N13/00C12N2533/78C12N2533/20
Inventor 王书华孔颖刘锋李刚苏绚涛桑元华刘宏
Owner SHANDONG UNIV
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