Application of KRP gene to pest control

A gene and gene expression technology, applied in the application field of KRP gene in pest control, can solve problems such as unclear
CN112062824AActive Publication Date: 2020-12-11SOUTH CHINA NORMAL UNIVERSITY
1 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA NORMAL UNIVERSITY
Publication Date
2020-12-11

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to the technical field of gene engineering, in particular to application of a KRP gene in pest control. According to the application provided by the invention, through construction of a CRISPR / Cas9 knockout strain of silkworm BmKRP, the influence of a new transcription factor BmKRP on the reproductive process of female silkworms is preliminarily explored and studied; and through observation on the silkworm BmKRP<- / -> phenotype, the effect that the deletion of BmKRP influences the female reproduction of silkworms, resulting in ovarian reduction, oocyte volume reduction andadult egg laying number reduction is found; and the effects that the mutation deletion of BmKRP influences the combination of BmKRP with reaction elements on a BmKrh1 promoter, so that BmKr-h1 does not express or down-regulates the expression, and important significance is realized on explaining a molecular mechanism of the silkworm hormone regulation reproduction are found. Through study, a similar control mechanism existing in other insects is speculated. Clues and theoretical guidance are provided for beneficial insect utilization, and pest control new targets and new strategies, and the application significance is realized.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to the application of KRP gene in pest control. Background technique

[0002] The silkworm (Bombyx mori L.) is a silk-producing insect of the order Lepidoptera, which belongs to invertebrates, and is a species of silkworm in the family Bombyxidae Arthropodae. In the field of scientific research, silkworm, as an important model insect of Lepidoptera, is widely used to study insect reproductive genetics and metamorphosis, so as to grasp and use its growth regulation mechanism, and finally achieve the effect of agricultural and forestry pest control.

[0003] Bombyx mori is a holometabolous insect. One generation has to go through four completely different developmental stages: egg, larva, pupa and adult; its larvae need to go through five molts in the stage of turning into pupa. The process of molting and metamorphosis of silkworm is regulated by complex hormone endocrine...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More