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Method capable of maintaining spore activity in bacteria solution of plate long spore bacteria

A technology of long spores and spores, which is applied in the field of maintaining the spore viability in the bacterial solution of the long spores, can solve the problems of the decline of bacterial activity and the short storage time, and achieve the effect of long storage time.

Pending Publication Date: 2020-12-11
瑞楚生物科技(江苏)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the bacterial viability will decrease during transportation in the form of bacterial liquid, and the storage time is short

Method used

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  • Method capable of maintaining spore activity in bacteria solution of plate long spore bacteria
  • Method capable of maintaining spore activity in bacteria solution of plate long spore bacteria
  • Method capable of maintaining spore activity in bacteria solution of plate long spore bacteria

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Embodiment Construction

[0017] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0018] The invention provides a technical solution: a method for maintaining the viability of spores in the bacterium of Discospora bacterium. Firstly, a vacuum bag made of nylon flexible plastic material is used to fill the bacterium solution.

[0019] The bacterial liquid will continue to grow and reproduce during transportation and storage, and waste gas such as carbon dioxide will be generated. If glass or plastic bottles are used to fill the bacterial liquid, it is likely that the bottle will be deformed or even burst during tra...

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Abstract

The invention discloses a method capable of maintaining spore activity in a bacterial solution of plate long spore bacteria. The method comprises the following steps of S1, putting a culture medium into a flexible plastic vacuum bag, carrying out irradiation sterilization, and filling a plate long spore bacteria fermentation bacterial solution into the flexible plastic vacuum bag; and S2, when thestrain fermentation end point is reached, providing the culture medium again. According to the method, the problem that the activity of strains is reduced during transportation in a bacterial solution form is solved, and meanwhile, the storage time is long.

Description

technical field [0001] The invention relates to the technical field of Discochospora bacterial liquid, in particular to a method capable of maintaining the viability of spores in the Discochospora bacterial liquid. Background technique [0002] Discochospora is a dodder pathogenic fungus. It can infect soybean dodder to make it sick and die, so as to achieve the purpose of controlling this weed. This bacterium was first discovered and applied by the Institute of Plant Protection, Shandong Academy of Agricultural Sciences, China. Soybean Cuscuta is a vicious parasitic weed that harms soybeans. It is widely distributed in major soybean-producing areas in China. Generally, the damage can reduce yield by 10-20%, and severe flakes will fail to harvest. Using Lubao No. 1 to control soybean dodder, the control effect is generally above 85%. The conidia of Shandong, Jiangsu, Anhui, Ningxia, Shaanxi, Hubei, Xinjiang, Hebei, Henan, and Lubao No. 1 were long oval, colorless, and sin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/04C12R1/645
CPCC12N1/14C12N1/04
Inventor 姜自军蔡蓓蓓朱琳徐绎涵仝光杰王文伟
Owner 瑞楚生物科技(江苏)有限公司
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