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Primer group for constructing broad bean fingerprint spectrum, fingerprint spectrum and application

A fingerprint and primer set technology, which is applied to the construction of broad bean fingerprint primer sets and fingerprints and application fields, can solve the problems of being easily affected by the environment, unfavorable large-scale seed breeding, and inaccurate identification of quantitative traits, etc., to achieve operational convenient effect

Active Publication Date: 2020-12-11
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, morphology is usually used to identify different breeding nurseries in the breeding process of original species. However, traditional phenotypic identification is effective for qualitative traits, but not accurate for quantitative traits. It is easily affected by the environment and the identification period is mostly in the mature stage. , not conducive to large-scale seed propagation

Method used

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  • Primer group for constructing broad bean fingerprint spectrum, fingerprint spectrum and application
  • Primer group for constructing broad bean fingerprint spectrum, fingerprint spectrum and application
  • Primer group for constructing broad bean fingerprint spectrum, fingerprint spectrum and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) SSR method for purity identification

[0059] Using 33 pairs of primers that were screened out and suitable for the identification of the purity of broad beans, the consistency of the original population of TF39 (the broad bean strain bred by Vicia fresh broad bean and Qingcan No. 14, whose pedigree is shown in Table 1) was analyzed, The sequences of the 33 pairs of primers used are shown in Table 2:

[0060] Table 1 Pedigree diagram

[0061] years Breeding generation Combination (code name) 2002 configuration combination 130×166(0220) 2003 F1 Mixed collection(0220) 2004-2005 F2, F3 Hybrid selection (0220) 2006 F4 Selection of excellent single plant (0220-4) 2007 F5 Selection of excellent individual plants (0220-4-3) 2008-2011 F6 Mixed selection, (0220-4-3) 2012-2016 Mixed, code name: TF39 2017 Yield identification comparison; code name TF39 2018 regional test

[0062] Table 2 Name...

Embodiment 2

[0077] (1) SSR for plant row identification

[0078] Utilize 33 pairs of primers in Example 1 to identify the strain line: according to the actual demand of the actual purity requirement of more than 95% in production, with a similarity coefficient of 0.95 as the standard, the strain line is identified according to the similarity coefficient based on the SSR molecular marker in Table 3 A total of 70 strain lines were screened out (marked with "*" in Table 3). Used for the identification of the next step between lines.

[0079] In Table 3, the genetic similarity coefficient s=ns / (ns+nd), where ns represents the number of traits that both germlines have, and nd represents the number of traits in one germline. Morphology was investigated by traits, quantified as presence and absence as 0 or 1, and molecular markers were read according to the bands.

[0080] Table 3 Comparison of genetic similarity coefficients between individuals in a row

[0081]

[0082]

[0083] Utili...

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PUM

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Abstract

The invention discloses a primer group for constructing a broad bean fingerprint spectrum, the fingerprint spectrum and application, and belongs to the technical field of molecular biology. 17 pairs of primer pairs for constructing the broad bean fingerprint spectrum are screened out, and the broad bean fingerprint spectrum is constructed on the basis of the 17 pairs of primer pairs and is appliedto purity identification of broad bean hybrids. The primer group has the advantages of being rapid, accurate, convenient to operate and the like, and the identification result is not affected by theenvironment.

Description

technical field [0001] The invention relates to the technical field of molecular biology, and more specifically relates to a primer set for constructing broad bean fingerprints, fingerprints and applications. Background technique [0002] Broad beans are the third most important winter food bean crop in the world. The nutritional value of broad beans is high, and its protein content is 25-35%. Broad beans are also rich in sugar, minerals, vitamins, calcium and iron. As a nitrogen-fixing crop, broad beans can convert molecular nitrogen in nature into nitrogen compounds and increase soil nitrogen content. Broad beans belong to small miscellaneous grains and have very important value in life. It can be used not only as a traditional ration, but also as a modern green food and nutritional health food. It is a food crop and animal feed rich in nutrition and protein. [0003] Seeds are the main means of agricultural production. Seed purity is the core index of seed quality, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 刘玉皎滕长才陈扣梅侯万伟李萍丁宝军
Owner QINGHAI UNIVERSITY
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