Preparation method of high-viscosity sliced paraffin

Abstract

The invention discloses a preparation method of high-viscosity sliced paraffin. The preparation method comprises the following steps: adding 100-200 parts of microcrystalline wax into 750 parts of fully refined paraffin; emulsifying, precipitating and filtering to obtain a filtrate, namely a high-purity paraffin liquid; adding 3-6 parts of butyl rubber into 100 parts of fully-refined paraffin, andheating for dissolving; completely dissolving butyl rubber in paraffin to prepare an auxiliary material; and adding auxiliary materials into the filtrate, fully stirring, granulating when the temperature is reduced to 75 DEG C, and curing to obtain the high-viscosity sliced paraffin. Due to the addition of a small amount of butyl rubber, cohesion of paraffin is greatly improved due to a macromolecular reticular structure, the paraffin has excellent elasticity due to the unique structure, the paraffin is prevented from cracking during low-temperature freezing, and the paraffin is prevented from scattering, peculiar smell and precipitation during high-temperature spreading.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for making high-viscosity section paraffin. Background technique [0002] Paraffin section is the most widely used method in routine histological preparation techniques. Paraffin section is not only used to observe the morphological structure of normal cells and tissues, but also the main method for studying, observing and judging the morphological changes of cells and tissues in pathology and forensic science, and has been widely used in research in many other disciplines middle. Paraffin sections include the steps of sampling, fixing, washing and dehydration, clearing, paraffin immersion, embedding, sectioning and mounting, dewaxing, staining, dehydration, clearing, and mounting. [0003] Pathological film production is one of the important links in pathological diagnosis. The quality of film production directly affects the accuracy and timeliness of pathologica...

AI Technical Summary

Problems solved by technology

At present, the paraffin wax produced in China has poor embedding effect, and it is easy to damage the cutter when slicing, and it is easy to disperse the slices when the slices are spread at high temperature (also known as unfolding slices), and the scrap rate of slices is high.

At present, domestic people generally pay attention to how to improve the sectioning method to obtain high-quality sections. At present, there is no way to increase the viscosity of paraffin itself to improve the quality of sections.

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