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Calibrator for fructosamine detection and detection kit using calibrator

A detection kit and fructosamine technology, applied in the field of biological detection, can solve the problems of change in detection value, slowness, difficult extraction of glycated serum proteins, etc., and achieve the effects of low cost, accurate detection results, and good transportation stability.

Pending Publication Date: 2020-12-11
海丰生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the above detection method, the standard substance is DMF, and DMF is a small molecular substance, which cannot represent the true structure of human serum fructosamine (that is, glycated serum protein is a macromolecular substance)
Glycated serum protein is difficult to extract, bovine serum albumin and glucose artificially synthesize fructosamine, because this non-enzymatic reaction is a slow process, its detection value will change with time, so it is not suitable for calibrator

Method used

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  • Calibrator for fructosamine detection and detection kit using calibrator
  • Calibrator for fructosamine detection and detection kit using calibrator
  • Calibrator for fructosamine detection and detection kit using calibrator

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1. Calibrators and kits for the detection of fructosamine

[0022] The embodiment of the present invention provides a new calibrator bovine serum albumin for the detection of fructosamine. Specifically, the present embodiment is based on the determination of fructosamine (FA) by using nitro blue tetrazolium chloride (NBT) reduction method with 1-deoxy-1-morpholine fructose (DMF) as the primary standard, and reasonably The determination of fructosamine (FA) with bovine serum albumin as the secondary standard was established, and the performance of the secondary standard was observed and analyzed experimentally. Among them, the English name of 1-deoxy-1-morpholine fructose is DEOXY-1-MORPHOLINO-D-FRUCTOSE, referred to as DMF, which is similar to fructosamine in terms of chemical structure and reaction with NBT.

[0023] The embodiment of the present invention uses bovine serum albumin as the calibrator (three grades) prepared as the main raw material to measur...

Embodiment 2

[0031] Embodiment 2, 1-deoxy-1-morpholine fructose (DMF) calibrator standardizes bovine serum albumin calibrator of the present invention

[0032] The sample detection accuracy test was carried out on the calibrator bovine serum albumin (tertiary) prepared by the present invention and the primary standard prepared with 1-deoxy-1-morpholine fructose (DMF).

[0033] 1. Experimental materials: calibrator-1: 1-deoxy-1-morpholine-D-fructose (0.28mmol / L), proclin-300 (0.03ml / L), assigned concentration: 280μmol / L; calibrator-2 : BSA (50g / L), proclin-300 (0.03ml / L), assigned concentration: 285μmol / L; glycosylated serum protein (NBT method) assay kit (Haifeng Biotechnology (Biology) Co., Ltd.).

[0034] 2. Experimental method: Calibrator-1 and calibrator-2 were respectively used to calibrate the glycated serum protein (NBT method) assay kit, and 100 serum samples were tested (abnormal samples were not less than 10%).

[0035] 3. Measurement results, as shown in Table 3, the test resul...

Embodiment 3

[0042] Embodiment 3, the transport stability test of the bovine serum albumin calibrator of the embodiment of the present invention

[0043] 1. Experimental materials: calibrator-2: BSA (50g / L), proclin-300 (0.03ml / L); glycosylated serum protein (NBT method) assay kit (Haifeng Biotechnology (Biology) Co., Ltd.).

[0044] 2. Experimental method: Take a calibrator-2 and put it in a 42°C incubator, take it out for testing on the third day, and test it together with the calibrator-2 on day 0.

[0045] 3. Experimental measurement results, as shown in Table 4, calibrator-2 accelerated stability.

[0046] Table 4

[0047]

[0048]4. Conclusion: From the test results above, it can be seen that the calibrator-2 prepared with bovine serum albumin as the main component has qualified for the stability of accelerated 3-day transport at 42°C.

[0049] In the determination of fructosamine (FA) by the nitro blue tetrazolium chloride (NBT) reduction method, the bovine serum albumin calibr...

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Abstract

The invention discloses a calibrator for fructosamine detection and a detection kit applying the calibrator. The calibrator is bovine serum albumin. The invention also discloses a detection kit for determining fructosamine. The detection kit adopts the bovine serum albumin as the calibrator. According to the invention, on the basis of using 1-deoxy-1-morpholine fructose (DMF) as a first-grade standard substance and using a nitrotetrazolium blue chloride (NBT) reduction method to determine the fructosamine (FA), the method for determining the fructosamine (FA) by using bovine serum albumin as the calibrator (third grade) is reasonably established; the method provided by the invention can make up for defects of DMF as a standard substance in aspects of a molecular structure, a reaction rateand reaction condition sensitivity; and meanwhile, advantages of an accurate sample detection result, low cost and better transportation stability are possessed.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a calibrator for the detection of fructosamine and a detection kit using the same. Background technique [0002] Fructosamine, also known as glycosylated serum protein, is a substance formed by protein in plasma during the non-enzymatic saccharification of glucose. Since the half-life of plasma protein is 17 days, fructosamine can reflect the blood sugar levels of diabetic patients within 1 to 3 weeks before testing. Average blood sugar level. To a certain extent, it makes up for the deficiency that glycosylated hemoglobin cannot reflect the change of blood sugar concentration in a short period of time. Determination of fructosamine is rapid and inexpensive (chemical method) and is a good indicator of diabetes control. [0003] Since Johnson established the nitroblue tetrazolium (NBT) reduction method for the determination of fructosamine (FA) in 1982, it has been ...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31G01N2201/127
Inventor 刘芳芳石怀兴温志国
Owner 海丰生物科技(北京)有限公司
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